製品の概要

  • 製品名Anti-Placental lactogen antibody
    Placental lactogen 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Placental lactogen
  • アプリケーション適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Purified human placental lactogen

  • ポジティブ・コントロール
    • Placenta

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーpH: 7.40
    Preservative: 0.1% Sodium azide
    Constituents: 0.0268% PBS, BSA
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab15554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
IHC-P 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Produced only during pregnancy and is involved in stimulating lactation, fetal growth and metabolism. Does not interact with GHR but only activates PRLR through zinc-induced dimerization.
  • 配列類似性Belongs to the somatotropin/prolactin family.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Choriomammotropin antibody
    • Chorionic somatomammotropin A antibody
    • chorionic somatomammotropin B antibody
    • chorionic somatomammotropin hormone 1 (placental lactogen) antibody
    • Chorionic somatomammotropin hormone 1 antibody
    • chorionic somatomammotropin hormone 2 antibody
    • Chorionic somatomammotropin hormone antibody
    • CS-2 antibody
    • CS1 antibody
    • CSA antibody
    • CSB antibody
    • CSH_HUMAN antibody
    • CSH1 antibody
    • CSH2 antibody
    • CSMT antibody
    • hCS-B antibody
    • hCSA antibody
    • Lactogen antibody
    • PL antibody
    • PL1 antibody
    • Placental lactogen antibody
    see all

Anti-Placental lactogen antibody 画像

  • ab15554 staining Human placenta by Immunohistochemistry (FFPE-sections).

  • Anti-Placental lactogen antibody (ab15554) at 1/100 dilution + Human recombinant hPL at 0.1 µg

    Secondary
    HRP conjugated donkey anti-rabbit IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
    Additional bands at : 10 kDa,14 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 5 minutes

    This image is courtesy of an Abreview submitted by Dr Stoil Dmitrov

    See Abreview

  • ab15554 at 1/200 staining human BeWo (placenta choriocarcinoma) cells by ICC/IF. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 30 minutes. A rhodamine conjugated goat anti-rabbit antibody was used as the secondary. The left hand panel of the image shows DAPI counterstain.

    See Abreview

  • Anti-Placental lactogen antibody (ab15554) at 1/25 dilution + Human placenta lysate

    Predicted band size : 25 kDa
    Observed band size : 23 kDa (why is the actual band size different from the predicted?)

Anti-Placental lactogen antibody (ab15554) 使用論文

This product has been referenced in:
  • Drukker M  et al. Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells. Nat Biotechnol 30:531-42 (2012). ICC/IF ; Human . Read more (PubMed: 22634564) »
  • Adachi K  et al. Role of SOX2 in maintaining pluripotency of human embryonic stem cells. Genes Cells : (2010). Read more (PubMed: 20384793) »

See all 3 Publications for this product

Product Wall

Unfortunately the PI of the antibody is not known.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (BeWo, placenta, choriocarcinoma)
Specification BeWo, placenta, choriocarcinoma
Fixative Paraformaldehyde
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1%
Username

Dr. Stoil Dmitrov

Verified customer

投稿 Apr 11 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Human recombinant hPL)
Loading amount 0.1 µg
Specification Human recombinant hPL
Blocking step Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Stoil Dmitrov

Verified customer

投稿 Apr 11 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"