Anti-Placental alkaline phosphatase (PLAP) 抗体 [SP15] (ab16695)

製品の概要

  • 製品名
    Anti-Placental alkaline phosphatase (PLAP) antibody [SP15]
    Placental alkaline phosphatase (PLAP) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [SP15] to Placental alkaline phosphatase (PLAP)
  • アプリケーション
    適用あり: IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Recombinant full length protein (Human).

  • エピトープ
    Not determined
  • ポジティブ・コントロール
    • Seminoma and placenta
  • 特記事項


    This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.1% Sodium Azide
    Constituents: Tissue culture supernatant
  • 精製度
    Tissue culture supernatant
  • 一次抗体 備考
    This antibody reacts with a membrane-bound isoenzyme of placental alkaline phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is useful in the identification of testicular germ cell tumors. Unlike germ cell tumors, PLAP-positive somatic cell tumors uniformly express epithelial membrane antigen (EMA).
  • ポリ/モノ
    モノクローナル
  • クローン名
    SP15
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab16695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

WB Use at an assay dependent concentration. PubMed: 16424182

ターゲット情報

  • 配列類似性
    Belongs to the alkaline phosphatase family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Alkaline phosphatase antibody
    • Alkaline phosphatase placental antibody
    • Alkaline phosphatase placental type antibody
    • Alkaline phosphatase Regan isozyme antibody
    • ALP antibody
    • Alp1 antibody
    • ALPP antibody
    • FLJ61142 antibody
    • Germ-cell alkaline phosphatase antibody
    • nagao isozyme antibody
    • OTTHUMP00000164354 antibody
    • PALP antibody
    • Placental alkaline phosphatase 1 antibody
    • placental heat-stable alkaline phosphatase antibody
    • placental type antibody
    • PLAP antibody
    • PLAP-1 antibody
    • PLAP1 antibody
    • PPB1_HUMAN antibody
    see all

画像

  • Human seminoma stained with ab16695.
  • Immunohistochemical analysis of paraffin-embedded Human seminoma tissue labelling Alkaline Phosphatase with ab137879 at 1/50 dilution.

参考文献

This product has been referenced in:
  • Pang HB  et al. Virion stiffness regulates immature HIV-1 entry. Retrovirology 10:4 (2013). Read more (PubMed: 23305456) »
  • Saffarini CM  et al. Induction and persistence of abnormal testicular germ cells following gestational exposure to di-(n-butyl) phthalate in p53-null mice. J Androl 33:505-13 (2012). IHC-P ; Mouse . Read more (PubMed: 21868749) »

See all 6 Publications for this product

レビューと Q&A

Thank you for your enquiry.

Here are the answers to your questions:

1. ab16517 - immunogen is Full length native protein (purified) (Human) and epitope has not been mapped
2. ab16695 - immunogen is Full length native protein (p...

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Please see the western blot in following reference noted under the 'References' section of the online datasheet for ab16695: Taylor DD et al. Pregnancy-associated exosomes and their modulation of T cell signaling. J Immunol 176:1534-42 (2006). WB; ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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