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Our Abpromise guarantee covers the use of ab99466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 153 kDa.|
ab99466 staining PLA2R in dog kidney tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked for 10 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + BSA) for 17 hours at 4°C. An Alexa Fluor® 568-conjugated rabbit anti-goat IgG polyclonal (1/500) was used as the secondary antibody.
Western Blot analysis showing approximate 150kDa band observed in Human Kidney and Pig Lung lysates.
ab99466 has not yet been referenced specifically in any publications.