製品の概要
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製品名Anti-PLA2R antibody
PLA2R 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to PLA2R
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由来種Goat
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特異性ab99466 is expected to recognize both isoforms (NP_031392.3; NP_001007268.1).
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アプリケーション適用あり: IHC-Fr, WBmore details
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種交差性交差種: Dog, Human, Pig
交差が予測される動物種: Rabbit, Cow, Orangutan
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免疫原
Synthetic peptide:
C-EKKKDTPKQHGT
, corresponding to internal sequence amino acids 946-957 of Human PLA2R (NP_031392.3; NP_001007268.1). -
ポジティブ･コントロール
- Human Ileum lysate.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）ab99466 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab99466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 153 kDa. |
ターゲット情報
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機能Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B.
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組織特異性Present in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level.
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配列類似性Contains 8 C-type lectin domains.
Contains 1 fibronectin type-II domain.
Contains 1 ricin B-type lectin domain. -
ドメインC-type lectin domains 3-5 mediate the interaction with phospholipase PLA2G1B.
The endocytosis signal probably mediates endocytosis via clathrin-coated pits. -
翻訳後修飾The secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form.
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細胞内局在Secreted and Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 317777 Cow
- Entrez Gene: 488367 Dog
- Entrez Gene: 22925 Human
- Entrez Gene: 100173143 Orangutan
- Entrez Gene: 100009180 Rabbit
- Omim: 604939 Human
- SwissProt: P49259 Cow
- SwissProt: Q13018 Human
see all -
別名
- 180 kDa secretory phospholipase A2 receptor antibody
- C-type lectin domain family 13 member C antibody
- CLEC13C antibody
see all
画像
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Immunohistochemistry (Frozen sections) - Anti-PLA2R antibody (ab99466)This image is courtesy of an anonymous Abreviewab99466 staining PLA2R in dog kidney tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked for 10 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + BSA) for 17 hours at 4°C. An Alexa Fluor® 568-conjugated rabbit anti-goat IgG polyclonal (1/500) was used as the secondary antibody.
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Western blot - Anti-PLA2R antibody (ab99466)Western Blot analysis showing approximate 150kDa band observed in Human Kidney and Pig Lung lysates.
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Western blot - Anti-PLA2R antibody (ab99466)Anti-PLA2R antibody (ab99466) at 1 µg/ml + Human Ileum lysate at 35 µg
Predicted band size: 153 kDa
参考文献
ab99466 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"