製品の概要

  • 製品名Anti-PLA2R antibody
    PLA2R 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to PLA2R
  • 特異性ab99466 is expected to recognize both isoforms (NP_031392.3; NP_001007268.1).
  • アプリケーション適用あり: IHC-Fr, WBmore details
  • 種交差性
    交差種: Dog, Human, Pig
    交差が予測される動物種: Rabbit, Cow, Orangutan
  • 免疫原

    Synthetic peptide:

    C-EKKKDTPKQHGT

    , corresponding to internal sequence amino acids 946-957 of Human PLA2R (NP_031392.3; NP_001007268.1).

  • ポジティブ・コントロール
    • Human Ileum lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab99466 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 153 kDa.

ターゲット情報

  • 機能Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B.
  • 組織特異性Present in lung macrophage (at protein level). Highly expressed in kidney. Also expressed in pancreas, amnion, choriodecidua and placenta. Isoform 2 is expressed at much lower level.
  • 配列類似性Contains 8 C-type lectin domains.
    Contains 1 fibronectin type-II domain.
    Contains 1 ricin B-type lectin domain.
  • ドメインC-type lectin domains 3-5 mediate the interaction with phospholipase PLA2G1B.
    The endocytosis signal probably mediates endocytosis via clathrin-coated pits.
  • 翻訳後修飾The secretory phospholipase A2 receptor form may be produced by the action of metalloproteinases. It contains all extracellular domains and only lacks transmembrane and cytosolic regions. It is however unclear whether this form is produced by proteolytic cleavage as suggested by some experiments, or by alternative splicing, as in the case of isoform 2 that shares all characteristics of secretory phospholipase A2 receptor form.
  • 細胞内局在Secreted and Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 180 kDa secretory phospholipase A2 receptor antibody
    • C-type lectin domain family 13 member C antibody
    • CLEC13C antibody
    • M type receptor antibody
    • M-type receptor antibody
    • Phospholipase A2 receptor 1 antibody
    • PLA2 R antibody
    • PLA2-R antibody
    • PLA2G1R antibody
    • PLA2IR antibody
    • PLA2R antibody
    • PLA2R_HUMAN antibody
    • PLA2R1 antibody
    • Soluble PLA2-R antibody
    • Soluble PLA2R antibody
    • Soluble secretory phospholipase A2 receptor antibody
    see all

Anti-PLA2R antibody 画像

  • ab99466 staining PLA2R in dog kidney tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with acetone and blocked for 10 minutes at 25°C. Samples were incubated with primary antibody (1/100 in PBS + BSA) for 17 hours at 4°C. An Alexa Fluor® 568-conjugated rabbit anti-goat IgG polyclonal (1/500) was used as the secondary antibody.

    See Abreview



  • Predicted band size : 153 kDa
    Observed band size : 150 kDa (why is the actual band size different from the predicted?)

    Western Blot analysis showing approximate 150kDa band observed in Human Kidney and Pig Lung lysates.

  • Anti-PLA2R antibody (ab99466) at 1 µg/ml + Human Ileum lysate at 35 µg

    Predicted band size : 153 kDa

Anti-PLA2R antibody (ab99466) 使用論文

ab99466 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step Block Ace as blocking agent for 10 minute(s) · Concentration: 4% · Temperature: 25°C
Sample Dog Tissue sections (kidney)
Specification kidney
Permeabilization No
Fixative Acetone
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投稿 Jun 23 2014

Thank you for contacting us and your interest in our products.

Unfortunately, we do not currently have any antibodies directed against PLA2R which have shown to detect the protein in mouse samples. I have checked the immunogen used to raise t...

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Thank you for contacting us. I can confirm that the immunogen sequences for these two antibodies are different. The immunogen for ab99466 is from an internal domain (amino acid 946 - 957), whereas the immunogen for ab80054 is from a C terminal doma...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"