製品の概要

  • 製品名Anti-PKN antibody [EPR3238]
    PKN 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR3238] to PKN
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to residues near the C terminus of Human PKN.

  • ポジティブ・コントロール
    • SH-SY-5Y, U937 and Jurkat cell lysates
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EPR3238
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab108973 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 104 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC,IHC-P or IP.
  • ターゲット情報

    • 関連性PKN belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis.
    • 細胞内局在Cytoplasmic
    • 参照データベース
    • 別名
      • DBK antibody
      • DPKN antibody
      • PAK 1 antibody
      • PRK 1 antibody
      • PROTEIN KINASE C RELATED KINASE 1 antibody
      • SERINE/THREONINE PROTEIN KINASE N antibody
      see all

    Anti-PKN antibody [EPR3238] 画像

    • All lanes : Anti-PKN antibody [EPR3238] (ab108973) at 1/1000 dilution

      Lane 1 : SH-SY-5Y cell lysates
      Lane 2 : U937 cell lysates
      Lane 3 : Jurkat cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 104 kDa
      Observed band size : 120 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of PKN in permeabilized Jurkat cells, using a 1/10 dilution of ab108973 (red) or a rabbit IgG (negative) (green).

    Anti-PKN antibody [EPR3238] (ab108973) 使用論文

    ab108973 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab108973.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"