製品の概要

  • 製品名Anti-PKM2 antibody
    PKM2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PKM2
  • アプリケーション適用あり: WB, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Monkey
  • 免疫原

    Synthetic peptide: L IAREAEAA IY HLQLFEE LRR LAPITS DPTE ATAVG AVEASFKCCS GAIIV LTKS GRSAHQ conjugated to KLH, corresponding to amino acids 380-440 of Human PKM2

  • ポジティブ・コントロール
    • Tonsil tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab85542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 58 kDa.
IHC-P 1/100.
IHC-Fr 1/100.

ターゲット情報

  • 機能Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • 組織特異性Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • パスウェイCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • 配列類似性Belongs to the pyruvate kinase family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • 細胞内局在Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CTHBP antibody
    • Cytosolic thyroid hormone binding protein antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • MGC3932 antibody
    • OIP 3 antibody
    • OIP-3 antibody
    • OIP3 antibody
    • OPA interacting protein 3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • PK muscle type antibody
    • PK, muscle type antibody
    • PK2 antibody
    • PK3 antibody
    • PKM antibody
    • PKM2 antibody
    • pykm antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase 3 antibody
    • Pyruvate kinase isozymes M1/M2 antibody
    • Pyruvate kinase muscle antibody
    • Pyruvate kinase muscle isozyme antibody
    • pyruvate kinase PKM antibody
    • Pyruvate kinase, muscle 2 antibody
    • TCB antibody
    • THBP1 antibody
    • Thyroid hormone binding protein 1 antibody
    • Thyroid hormone binding protein cytosolic antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2 PK antibody
    • Tumor M2-PK antibody
    see all

Anti-PKM2 antibody 画像

  • ab85542, at 1/100 dilution, staining PKM2 in formalin-fixed paraffin-embedded human tonsil tissue by Immunohistochemistry.
  • Anti-PKM2 antibody (ab85542) at 1/1000 dilution + Recombinant human PKM2 protein (ab89364) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

Anti-PKM2 antibody (ab85542) 使用論文

ab85542 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"