製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to PKM2
  • 特異性
    The antibody is raised against pyruvate kinase isolated from rabbit muscle. Reactivity with specific PK (PKM1, PKLR) has not been assessed.
  • アプリケーション
    適用あり: ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Rabbit, Human
    交差が予測される動物種: Mammal
  • 免疫原

    Purified pyruvate kinase from Rabbit muscle.

  • ポジティブ・コントロール
    • Uterus tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab79070 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • 組織特異性
    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • パスウェイ
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • 配列類似性
    Belongs to the pyruvate kinase family.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • 細胞内局在
    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CTHBP antibody
    • Cytosolic thyroid hormone binding protein antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • MGC3932 antibody
    • OIP 3 antibody
    • OIP-3 antibody
    • OIP3 antibody
    • OPA interacting protein 3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • PK muscle type antibody
    • PK, muscle type antibody
    • PK2 antibody
    • PK3 antibody
    • PKM antibody
    • PKM2 antibody
    • pykm antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase 3 antibody
    • Pyruvate kinase isozymes M1/M2 antibody
    • Pyruvate kinase muscle antibody
    • Pyruvate kinase muscle isozyme antibody
    • pyruvate kinase PKM antibody
    • Pyruvate kinase, muscle 2 antibody
    • TCB antibody
    • THBP1 antibody
    • Thyroid hormone binding protein 1 antibody
    • Thyroid hormone binding protein cytosolic antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2 PK antibody
    • Tumor M2-PK antibody
    see all

画像

  • ab79070, at 5µg/ml, staining PKM2 in uterus tissue by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue.

参考文献

ab79070 has not yet been referenced specifically in any publications.

レビューと Q&A

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10000µg/mL · Temperature: 25°C
Sample
Human Cell (Cholangiocarcinoma cell)
Specification
Cholangiocarcinoma cell
Permeabilization
Yes - 0.25% TritonX-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 May 26 2014



J’ai contacté le laboratoire est malheureusement l’épitope d’ab79070 n’a pas été déterminé.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (ECR 293 cell line)
Specification
ECR 293 cell line
Fixative
Formaldehyde
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Dr. Ismail Hendaoui

Verified customer

投稿 Feb 04 2013

Merci pour votre appel de hier.

La laboratoire à pu me confirmer, que pour l'IP1 x 107 cellules ont été lysées et resuspendues en 0.2ml. Ensuite, ils ont ajouté de 0.5à 5 ul de cet anticorps et incub&...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録