製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PKM2
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Monkey
  • 免疫原

    Synthetic peptide corresponding to Human PKM2 aa 476-505 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 5315

  • ポジティブ・コントロール
    • WB: HeLa, PC-12, NIH/3T3 and Ramos cell lysates and mouse brain tissue lysate. IHC-P: Human breast carcinoma and hepatocarcinoma tissues. ICC/IF: MCF7 and HeLa cells.
  • 特記事項

    There are 4 isozymes of pyruvate kinase in mammals: L, R, M1 and M2. ab38237 recognizes the M1/M2 isoform of pyruvate kinase.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38237 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
IHC-P 1/50 - 1/100.
WB 1/1000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).

ターゲット情報

  • 機能
    Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
  • 組織特異性
    Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
  • パスウェイ
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
  • 配列類似性
    Belongs to the pyruvate kinase family.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
    ISGylated.
  • 細胞内局在
    Cytoplasm. Nucleus. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic actvity.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CTHBP antibody
    • Cytosolic thyroid hormone binding protein antibody
    • Cytosolic thyroid hormone-binding protein antibody
    • KPYM_HUMAN antibody
    • MGC3932 antibody
    • OIP 3 antibody
    • OIP-3 antibody
    • OIP3 antibody
    • OPA interacting protein 3 antibody
    • Opa-interacting protein 3 antibody
    • p58 antibody
    • PK muscle type antibody
    • PK, muscle type antibody
    • PK2 antibody
    • PK3 antibody
    • PKM antibody
    • PKM2 antibody
    • pykm antibody
    • Pyruvate kinase 2/3 antibody
    • Pyruvate kinase 3 antibody
    • Pyruvate kinase isozymes M1/M2 antibody
    • Pyruvate kinase muscle antibody
    • Pyruvate kinase muscle isozyme antibody
    • pyruvate kinase PKM antibody
    • Pyruvate kinase, muscle 2 antibody
    • TCB antibody
    • THBP1 antibody
    • Thyroid hormone binding protein 1 antibody
    • Thyroid hormone binding protein cytosolic antibody
    • Thyroid hormone-binding protein 1 antibody
    • Tumor M2 PK antibody
    • Tumor M2-PK antibody
    see all

Anti-PKM2 antibody 画像

  • Anti-PKM2 antibody (ab38237) at 1/100 dilution + HeLa cell lysate

    Predicted band size : 58 kDa
    Observed band size : 58 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue labelling PKM2 with ab38237. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by DAB staining.

  • ICC/IF image of ab38237 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38237, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-PKM2 antibody (ab38237) + Ramos cell lysate at 35 µg

    Predicted band size : 58 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling PKM2 with ab38237 at 1/50. A peroxidase-conjugated anti-rabbit IgG was used as the secondary antibody, followed by ACE staining.

  • Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling PKM2 (green) with ab38237. Cells were fixed with 4% PFA (20 min) and permeabilized with Triton X-100 (0.2%, 30 min). Cells were incubated with the primary antibody (1/200, 2 h at room temperature). An Alexa Fluor® 488-conjugated donkey anti-rabbit antibody was used as the secondary antibody (1/1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 μg/ml, 5 min).

  • All lanes : Anti-PKM2 antibody (ab38237) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : Ramos cell lysate
    Lane 4 : NIH/3T3 cell lysate
    Lane 5 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 58 kDa
  • All lanes : Anti-PKM2 antibody (ab38237) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : PC-12 cell lysate
    Lane 3 : Ramos cell lysate
    Lane 4 : NIH/3T3 cell lysate
    Lane 5 : Mouse brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 58 kDa

Anti-PKM2 antibody (ab38237) 使用論文

This product has been referenced in:
  • Wei Y  et al. Pyruvate kinase type M2 promotes tumour cell exosome release via phosphorylating synaptosome-associated protein 23. Nat Commun 8:14041 (2017). Read more (PubMed: 28067230) »
  • Teng Y  et al. MicroRNA-29B (mir-29b) regulates the Warburg effect in ovarian cancer by targeting AKT2 and AKT3. Oncotarget 6:40799-814 (2015). IHC . Read more (PubMed: 26512921) »

See all 14 Publications for this product

Product Wall

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% Acrylamide gel)
Sample
Human Cell lysate - whole cell (MDA-MB-231 breast cancer cell line)
Specification
MDA-MB-231 breast cancer cell line
Treatment
TGF beta 2.5ng/ml-1 3 hrs
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Wendy Greenwood

Verified customer

投稿 Mar 23 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (liver)
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Nov 10 2014

Thank you for contacting Abcam.

I have been talking to my colleagues and we cannot think of any good ways to get an antibody to work in non-reduced, non-denatured conditions, they either work in that application or not.

I am sorry tha...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacementcontaining 1 vialeach of ab38237,13533,38295.

To check the status of the or...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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