Anti-PKLR 抗体 (ab96604)
Key features and details
- Rabbit polyclonal to PKLR
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PKLR antibody
PKLR 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKLR -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment, corresponding to a region within the N terminal amino acids 1-230 of Human PKLR.
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ポジティブ・コントロール
- 293T, A431, H1299, HeLa, HepG2, Raji cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96604の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 62 kDa.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 62 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Plays a key role in glycolysis. -
パスウェイ
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5. -
関連疾患
Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia. -
配列類似性
Belongs to the pyruvate kinase family. - Information by UniProt
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参照データベース
- Entrez Gene: 539579 Cow
- Entrez Gene: 5313 Human
- Entrez Gene: 18770 Mouse
- Entrez Gene: 24651 Rat
- Omim: 609712 Human
- SwissProt: P30613 Human
- SwissProt: P53657 Mouse
- SwissProt: P12928 Rat
see all -
別名
- EC 2.7.1.40 antibody
- KPYR_HUMAN antibody
- L-PK antibody
see all
画像
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All lanes : Anti-PKLR antibody (ab96604) at 1/5000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 62 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ab96604 at 1:200 dilution. Image 2: Merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab96604 は論文での使用が確認できていません。