製品の概要

  • 製品名Anti-PKLR antibody
    PKLR 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to PKLR
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length Human PKLR protein, amino acids 1-574 (NP_000289.1)

  • ポジティブ・コントロール
    • Human liver tissue lysate, PKLR transfected 293T cell lysate, Human kidney tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab89071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IHC-P
  • 追加情報IHC-P: Use at a concentration of 3 µg/ml. Antigen retrieval is recommended: Place sample in 1X citrate buffer (pH 6.0) and microwave at 750W for 20 minutes, cool sample
    WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Plays a key role in glycolysis.
    • パスウェイCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
    • 関連疾患Defects in PKLR are the cause of pyruvate kinase hyperactivity (PKHYP) [MIM:102900]; also known as high red cell ATP syndrome. This autosomal dominant phenotype is characterized by increase of red blood cell ATP.
      Defects in PKLR are the cause of pyruvate kinase deficiency of red cells (PKRD) [MIM:266200]. A frequent cause of hereditary non-spherocytic hemolytic anemia. Clinically, pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia.
    • 配列類似性Belongs to the pyruvate kinase family.
    • Information by UniProt
    • 参照データベース
    • 別名
      • EC 2.7.1.40 antibody
      • KPYR_HUMAN antibody
      • L-PK antibody
      • Pk-1 antibody
      • PK1 antibody
      • PKL antibody
      • Pklg antibody
      • Pklr antibody
      • PKR antibody
      • PKRL antibody
      • Pyruvate kinase 1 antibody
      • Pyruvate kinase isozymes R/L antibody
      • Pyruvate kinase liver and blood cell antibody
      • Pyruvate kinase liver and RBC antibody
      • Pyruvate kinase liver and red blood cell antibody
      • Pyruvate kinase liver type antibody
      • Pyruvate kinase type L antibody
      • Pyruvate kinase, red cell type antibody
      • R type/L type pyruvate kinase antibody
      • R-PK antibody
      • R-type/L-type pyruvate kinase antibody
      • Red cell/liver pyruvate kinase antibody
      • RPK antibody
      see all

    Anti-PKLR antibody 画像

    • Anti-PKLR antibody (ab89071) at 1 µg/ml + Human liver tissue lysate at 50 µg

      Predicted band size : 62 kDa
      Observed band size : 62 kDa
    • All lanes : Anti-PKLR antibody (ab89071) at 1 µg/ml

      Lane 1 : PKLR transfected 293T cell lysate
      Lane 2 : Non-transfected lysate

      Lysates/proteins at 25 µg per lane.


      Predicted band size : 62 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
    • ab89071 at 3µg/ml staining PKLR in Human kidney by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue.

    Anti-PKLR antibody (ab89071) 使用論文

    ab89071 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab89071.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"