Anti-PKC zeta (phospho T560) 抗体 (ab59412)
Key features and details
- Rabbit polyclonal to PKC zeta (phospho T560)
- Suitable for: WB, ELISA, IHC-P
- Reacts with: Human, African green monkey
- Isotype: IgG
製品の概要
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製品名
Anti-PKC zeta (phospho T560) antibody
PKC zeta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKC zeta (phospho T560) -
由来種
Rabbit -
特異性
Detects endogenous levels of PKC? only when phosphorylated at threonine 560. -
アプリケーション
適用あり: WB, ELISA, IHC-Pmore details -
種交差性
交差種: Human, African green monkey -
免疫原
Synthetic peptide corresponding to Human PKC zeta aa 500-600 (phospho T560).
Database link: Q05513 -
ポジティブ・コントロール
- WB: Extracts from COS7 cells treated with PMA (125ng/ml, 30mins). IHC-P: Human brain gliomas, human brain tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59412の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
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ELISA |
1/20000.
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IHC-P |
1/50 - 1/100.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa). |
ELISA
1/20000. |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization. -
組織特異性
Expressed in brain, and to a lesser extent in lung, kidney and testis. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
ドメイン
The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
The C1 domain does not bind the diacylglycerol (DAG). -
翻訳後修飾
CDH5 is required for its phosphorylation at Thr-410. -
細胞内局在
Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction. - Information by UniProt
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参照データベース
- Entrez Gene: 5590 Human
- Omim: 176982 Human
- SwissProt: Q05513 Human
- Unigene: 496255 Human
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別名
- 14-3-3-zetaisoform antibody
- AI098070 antibody
- aPKCzeta antibody
see all
画像
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All lanes : Anti-PKC zeta (phospho T560) antibody (ab59412) at 1/500 dilution
Lane 1 : Extracts from COS7 cells treated
with PMA (125ng/ml, 30mins)
Lane 2 : Extracts from COS7 cells treated
with PMA (125ng/ml, 30mins) with phosphopeptide
Predicted band size: 68 kDa
Observed band size: 68 kDa -
Immunohistochemical analysis of paraffin embedded human brain gliomas using ab59412 at 1/50 dilution
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Immunohistochemical analysis of paraffin embedded human brain tissue using ab59412 at 1/50 dilution, in the presence (right) and absence (left) of immunising phosphopeptide.
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ELISA showing that ab59412 (1:20000) detects endogenous levels of PKC zeta only when phosphorylated at Thr560.
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Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using PKC zeta (Phospho-Thr560) Antibody, ab59412.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab59412 は 12 報の論文で使用されています。
- Patel R et al. Simultaneous inhibition of atypical protein kinase-C and mTOR impedes bladder cancer cell progression. Int J Oncol 56:1373-1386 (2020). PubMed: 32236625
- Asim M et al. Ketamine Alleviates Fear Generalization Through GluN2B-BDNF Signaling in Mice. Neurosci Bull 36:153-164 (2020). PubMed: 31444653
- Taoro-Gonzalez L et al. Extracellular cGMP Reverses Altered Membrane Expression of AMPA Receptors in Hippocampus of Hyperammonemic Rats: Underlying Mechanisms. Mol Neurobiol 56:4428-4439 (2019). PubMed: 30328550
- Islam SMA et al. The modulation of actin dynamics via atypical Protein Kinase-C activated Cofilin regulates metastasis of colorectal cancer cells. Cell Adh Migr 13:106-120 (2019). PubMed: 30417717
- Taoro-Gonzalez L et al. Hyperammonemia alters membrane expression of GluA1 and GluA2 subunits of AMPA receptors in hippocampus by enhancing activation of the IL-1 receptor: underlying mechanisms. J Neuroinflammation 15:36 (2018). WB ; Rat . PubMed: 29422059