製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PKC zeta
  • 由来種
    Rabbit
  • 特異性
    ab59364 detects endogenous levels of total PKC zeta protein. The immunogen used for this product shares 89% homology with PKC iota/lambda. Cross-reactivity with this protein has not been confirmed experimentally.
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Xenopus laevis
  • 免疫原

    Synthetic peptide within Human PKC zeta aa 526-575. The exact sequence is proprietary. Synthesized non-phosphopeptide derived from human PKC zeta around the phosphorylation site of threonine 560 (Q-L-TP-P-D).
    Database link: Q05513

  • ポジティブ・コントロール
    • WB: Xenopus laevis cytoplasmic egg extract. PMA treated COS7 cell extracts. Rat hippocampus lysate. IP: PKC zeta IP in mouse brain tissue lysate. ICC/IF: NIH/3T3 cells. IHC-P: Human proliferative endometrial tissue. Human brain tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab59364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 68 kDa.
ELISA 1/1000.
IHC-P 1/50 - 1/100.
IP Use a concentration of 5 µg/ml.
ICC/IF 1/100 - 1/500.

ターゲット情報

  • 機能
    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
  • 組織特異性
    Expressed in brain, and to a lesser extent in lung, kidney and testis.
  • 配列類似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • ドメイン
    The OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
    The C1 domain does not bind the diacylglycerol (DAG).
  • 翻訳後修飾
    CDH5 is required for its phosphorylation at Thr-410.
  • 細胞内局在
    Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 14-3-3-zetaisoform antibody
    • AI098070 antibody
    • aPKCzeta antibody
    • C80388 antibody
    • EC 2.7.11.13 antibody
    • KPCZ_HUMAN antibody
    • nPKC zeta antibody
    • nPKC-zeta antibody
    • OTTHUMP00000001368 antibody
    • OTTHUMP00000044160 antibody
    • PKC 2 antibody
    • PKC ZETA antibody
    • PKC2 antibody
    • Pkcz antibody
    • PKCZETA antibody
    • PKM-zeta, included antibody
    • PRKCZ antibody
    • Protein kinase C zeta antibody
    • Protein kinase C zeta form antibody
    • Protein kinase C zeta type antibody
    • r14-3-3 antibody
    • R74924 antibody
    • zetaPKC antibody
    see all

画像

  • Anti-PKC zeta antibody (ab59364) at 1/500 dilution + Xenopus laevis cytoplasmic egg extract at 40 µg

    Secondary
    Goat polyclonal - IRDye® 800CW at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 68 kDa


    Exposure time: 5 seconds

    See Abreview

  • Immunohistochemical analysis of human proliferative endometrial tissue, staining PKC zeta with ab59364.

    Antigen retrieval was performed by heat mediation in a citrate buffer. Tissue was blocked with blocking solution before incubating with primary antibody (1/100) overnight at 4°C. Staining was detected using DAB.

  • Immunofluorescence analysis of NIH/3T3 cells using PKC zeta at dilution of 1:100.

  • PKC zeta was immunoprecipitated from 0.5 mg mouse brain tissue with 5 µg of ab59364 and 50 µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10 minutes; Mouse brain tissue lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 minutes under agitation.

    Proteins were eluted by addition of 40 µl SDS loading buffer and incubated for 10 minutes at 70°C; 10 µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab59364.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Anti-Rabbit IgG light chain HRP (ab99697).

    Band: 68kDa; PKC zeta, non specific bands - 52kDa: We are unsure as to the identity of this extra band.

  • All lanes : Anti-PKC zeta antibody (ab59364) at 1/500 dilution

    Lane 1 : Extracts from COS-7 (African green monkey kidney fibroblast-like cell line) cells, treated with PMA (125 ng/ml, 30mins), without immunizing peptide
    Lane 2 : Extracts from COS7 (African green monkey kidney fibroblast-like cell line)cells, treated with PMA (125 ng/ml, 30mins), plus immunizing peptide

    Predicted band size: 68 kDa
    Observed band size: 80 kDa (why is the actual band size different from the predicted?)

  • Lanes 2-4 : Anti-PKC zeta antibody (ab59364) at 1/500 dilution

    Lanes 1-2 : Rat hippocampus lysate at 40 µg
    Lanes 3-4 : Rat hippocampus lysate at 20 µg

    Secondary
    All lanes : Anti-Rabbit IR800-linked conjugation: IRDye® 800CW

    Performed under reducing conditions.

    Predicted band size: 68 kDa


    Exposure time: 5 minutes

    See Abreview

  • ab59364 at a 1/50 dilution staining PKC zeta in formalin/PFA fixed paraffin-embedded human brain tissue using Immunohistochemistry.

    Left panel: Without immunizing peptide.

    Right image: Plus immunizing peptide.

参考文献

This product has been referenced in:
  • Dong Z  et al. Long-term potentiation decay and memory loss are mediated by AMPAR endocytosis. J Clin Invest 125:234-47 (2015). Read more (PubMed: 25437879) »
  • Corriden R  et al. Tamoxifen augments the innate immune function of neutrophils through modulation of intracellular ceramide. Nat Commun 6:8369 (2015). Read more (PubMed: 26458291) »

See all 5 Publications for this product

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Xenopus laevis Cell (X. laevis early embryo (4 cell))
Specification
X. laevis early embryo (4 cell)
Fixative
DENTS (80% Methanol, 20% DMSO)
Permeabilization
Yes - PBS 0.1% Triton-X100
Blocking step
PBS 0.1% Triton-X100, 2 mg/ml BSA, 10% goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - other (Xenopus laevis cytoplasmic egg extract)
Loading amount
40 µg
Specification
Xenopus laevis cytoplasmic egg extract
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Apr 17 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (Hippocampus lysate)
Loading amount
40 µg
Specification
Hippocampus lysate
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Richard D'Mello

Verified customer

投稿 Apr 08 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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