Customer reviews and FAQs

製品の概要

  • 製品名Anti-PKC zeta antibody
    PKC zeta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PKC zeta
  • アプリケーション適用あり: WB, ELISA, IHC-Fr, ICC, IPmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mammal
  • 免疫原

    Synthetic peptide:

    EYINPLLLSAEESV

    , corresponding to amino acids 579-592 of Rat PKC zeta.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Rat brain and myeloid tissues.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Predicted molecular weight: 71 kDa. (using horseradish peroxidase or alkaline phosphatase).
ELISA Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.
ICC 1/250. Frozen. (using horseradish peroxidase or alkaline phosphatase).
IP 1/300. PubMed: 19028678

ターゲット情報

  • 機能This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization.
  • 組織特異性Expressed in brain, and to a lesser extent in lung, kidney and testis.
  • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 OPR domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 protein kinase domain.
  • ドメインThe OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
    The C1 domain does not bind the diacylglycerol (DAG).
  • 翻訳後修飾CDH5 is required for its phosphorylation at Thr-410.
  • 細胞内局在Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
  • Information by UniProt
  • 参照データベース
    • 別名
      • 14-3-3-zetaisoform antibody
      • AI098070 antibody
      • aPKCzeta antibody
      • C80388 antibody
      • EC 2.7.11.13 antibody
      • KPCZ_HUMAN antibody
      • nPKC zeta antibody
      • nPKC-zeta antibody
      • OTTHUMP00000001368 antibody
      • OTTHUMP00000044160 antibody
      • PKC 2 antibody
      • PKC ZETA antibody
      • PKC2 antibody
      • Pkcz antibody
      • PKCZETA antibody
      • PKM-zeta, included antibody
      • PRKCZ antibody
      • Protein kinase C zeta antibody
      • Protein kinase C zeta form antibody
      • Protein kinase C zeta type antibody
      • r14-3-3 antibody
      • R74924 antibody
      • zetaPKC antibody
      see all

    Anti-PKC zeta antibody 画像

    • Western blot - Anti-PKC zeta antibody (ab4137)
      Western blot - Anti-PKC zeta antibody (ab4137)Image from Barros SA et al, J Biol Chem. 2009 Jan 30;284(5):2672-9. Epub 2008 Nov 21, Fig 2.


      Predicted band size : 71 kDa

      Image from Barros SA et al, J Biol Chem. 2009 Jan 30;284(5):2672-9. Epub 2008 Nov 21, Fig 2.

      Immunoprecipitation.
      Rat renal cortex from adult male Sprague-Dawley CD rats was isolated and suspended in CelLyticTM MT Mammalian Tissue Lysis/Extraction reagent containing complete protease inhibitor mixture. Tissues were then homogenized and centrifuged at 2,500 x g for 10 minutes at 4 °C. The supernatant was used for immunoprecipitation. To eliminate nonspecific binding, 1 ml of rat kidney lysate (3 mg of protein) was pre-cleared by incubation with 50µl of TrueBlotTM anti-rabbit Ig IP beads overnight at 4 °C. Cleared cell lysates were incubated with either 7µg of ab4137 or 1µg of polyclonal rabbit anti-rOAT3 for 2 hours at 4 °C. TrueBlot anti-rabbit Ig IP beads (30µl) was added and incubation was continued for 2 hours at 4 °C. Bound proteins were washed 7 times with increasing washing stringency IP buffers and eluted from beads by heating in 4 x NuPAGE LDS sample buffer for 10 minutes. The Invitrogen NuPAGE Novex Tris

    Anti-PKC zeta antibody (ab4137) 使用論文

    This product has been referenced in:
    • Levy D  et al. Lysine methylation of the NF-?B subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-?B signaling. Nat Immunol 12:29-36 (2011). Read more (PubMed: 21131967) »
    • Barros SA  et al. Activation of protein kinase Czeta increases OAT1 (SLC22A6)- and OAT3 (SLC22A8)-mediated transport. J Biol Chem 284:2672-9 (2009). WB, IP ; Rat . Read more (PubMed: 19028678) »

    See all 3 Publications for this product

    ab4137 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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    ab4137 の abreview を投稿する ab4137 に関する問い合わせをする

    3 Abreviews or Q&A

    10
    Votes: Highest First

    Dear Madam/Sir,   I have recently read an interesting article regarding the regulatory role of PKCzeta isoform on F0F1 ATP synthase beta subunit. It claimed that PKCzeta is able to phosphorilate the beta subunit of the F0F1 ATP synthase and hence, r...

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    Thank you for your inquiry. I have conducted a search for you and found the following antibodies: ab43176 - Rabbit polyclonal to PKC zeta ab4137 - Chicken polyclonal to ATP Synthase beta ab51157 - Rabbit polyclonal to PKC zeta ab59412 - Rabb...

    Read More

    BATCH NUMBER 119876 DESCRIPTION OF THE PROBLEM I see an obvious band at 50 KD instead of around 71 KD. It is not at right size. SAMPLE Human cell extract PRIMARY ANTIBODY PKC zeta antibody (abcam/ rabbit/1:250/overnight incubation/3 ...

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    Thank you for your patience and for providing details regarding your protocol. Ab4137 was characterized for use in Western blotting with rat brain and myeloid tissues. This antibody has not yet been tested for cross-reactivity with human samples. I wou...

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    Customer is using this antibody in WB with rat samples. At a dilution of 1:250 and incubation overnight at 4C, there are no bands. Secondary is goat anti-rabbit at 1:1500 and is working with other primaries. Abcam lot# 105842.

    Thank you for your enquiry. Did you try increasing the concentration of the primary to 1:100? Please let me know if that improved your results. What specifically are the rat samples that you are using? Can you tell me as well, how did you store the ant...

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