製品の概要
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製品名Anti-PKC zeta antibody
PKC zeta 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to PKC zeta
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由来種Rabbit
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アプリケーション適用あり: WB, ELISA, IHC-Fr, ICC, IPmore details
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種交差性交差種: Rat
交差が予測される動物種: Mammal
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免疫原
Synthetic peptide:
EYINPLLLSAEESV
, corresponding to amino acids 579-592 of Rat PKC zeta. -
ポジティブ･コントロール
- Rat brain and myeloid tissues.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.05% Sodium Azide
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精製度Whole antiserum
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab4137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/250. Predicted molecular weight: 71 kDa. (using horseradish peroxidase or alkaline phosphatase). | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC | 1/250. Frozen. (using horseradish peroxidase or alkaline phosphatase). | |
| IP | 1/300. PubMed: 19028678 |
ターゲット情報
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機能This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Phosphorylates 'Ser-311' of RELA subunit of NF-kappa-B.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. Subunit of a quaternary complex that plays a central role in epithelial cell polarization. -
組織特異性Expressed in brain, and to a lesser extent in lung, kidney and testis.
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配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 OPR domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 protein kinase domain. -
ドメインThe OPR domain mediates mutually exclusive interactions with SQSTM1 and PARD6B.
The C1 domain does not bind the diacylglycerol (DAG). -
翻訳後修飾CDH5 is required for its phosphorylation at Thr-410.
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細胞内局在Cytoplasm. Endosome. Cell junction. In the retina, localizes in the terminals of the rod bipolar cells (By similarity). Associates with endosomes. Presence of KRIT1, CDH5 and RAP1B is required for its localization to the cell junction.
- Information by UniProt
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参照データベース
- Entrez Gene: 25522 Rat
- SwissProt: P09217 Rat
- Unigene: 1109 Rat
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別名
- 14-3-3-zetaisoform antibody
- AI098070 antibody
- aPKCzeta antibody
see all
画像
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Western blot - Anti-PKC zeta antibody (ab4137)Image from Barros SA et al, J Biol Chem. 2009 Jan 30;284(5):2672-9. Epub 2008 Nov 21, Fig 2.Immunoprecipitation.
Rat renal cortex from adult male Sprague-Dawley CD rats was isolated and suspended in CelLyticTM MT Mammalian Tissue Lysis/Extraction reagent containing complete protease inhibitor mixture. Tissues were then homogenized and centrifuged at 2,500 x g for 10 minutes at 4 °C. The supernatant was used for immunoprecipitation. To eliminate nonspecific binding, 1 ml of rat kidney lysate (3 mg of protein) was pre-cleared by incubation with 50µl of TrueBlotTM anti-rabbit Ig IP beads overnight at 4 °C. Cleared cell lysates were incubated with either 7µg of ab4137 or 1µg of polyclonal rabbit anti-rOAT3 for 2 hours at 4 °C. TrueBlot anti-rabbit Ig IP beads (30µl) was added and incubation was continued for 2 hours at 4 °C. Bound proteins were washed 7 times with increasing washing stringency IP buffers and eluted from beads by heating in 4 x NuPAGE LDS sample buffer for 10 minutes. The Invitrogen NuPAGE Novex Tris
プロトコール
参考文献
This product has been referenced in:
- Levy D et al. Lysine methylation of the NF-?B subunit RelA by SETD6 couples activity of the histone methyltransferase GLP at chromatin to tonic repression of NF-?B signaling. Nat Immunol 12:29-36 (2011). Read more (PubMed: 21131967) »
- Barros SA et al. Activation of protein kinase Czeta increases OAT1 (SLC22A6)- and OAT3 (SLC22A8)-mediated transport. J Biol Chem 284:2672-9 (2009). WB, IP ; Rat . Read more (PubMed: 19028678) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"