Anti-PKC theta (phospho S676) 抗体 (ab47774)


  • 製品名
    Anti-PKC theta (phospho S676) antibody
    PKC theta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PKC theta (phospho S676)
  • 特異性
    This antibody detects PKC theta only when phosphorylated at serine 676.
  • アプリケーション
    適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide corresponding to Human PKC theta aa 643-692 (C terminal).

  • ポジティブ・コントロール
    • IHC-P: Human breast carcinoma tissue. WB: JK cell extracts.



Our Abpromise guarantee covers the use of ab47774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
IHC-P Use at an assay dependent concentration.
ELISA 1/4000.


  • 機能
    This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 組織特異性
    Skeletal muscle, megakaryoblastic cells and platelets.
  • 配列類似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • ドメイン
    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain.
  • 翻訳後修飾
    Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KPCT_HUMAN antibody
    • MGC126514 antibody
    • MGC141919 antibody
    • nPKC theta antibody
    • nPKC-theta antibody
    • nPKCtheta antibody
    • OTTHUMP00000043364 antibody
    • OTTHUMP00000043365 antibody
    • PKC 0 antibody
    • PKC0 antibody
    • Prkcq antibody
    • PRKCT antibody
    • Protein kinase C theta antibody
    • Protein kinase C theta type antibody
    • Protein Kinase Ctheta antibody
    see all

Anti-PKC theta (phospho S676) antibody 画像

  • This image shows human breast carcinoma stained with ab47774 at 1/50 dilution. Right hand image shows tissue treated with the immunogenic phosphopeptide, whereas the left hand image is untreated.
  • All lanes : Anti-PKC theta (phospho S676) antibody (ab47774) at 1/500 dilution

    Lane 1 : JK cell extract treated with 200nM PMA for 30mins. No peptide.
    Lane 2 : JK cell extract, untreated. No peptide.

    Predicted band size : 82 kDa

Anti-PKC theta (phospho S676) antibody (ab47774) 使用論文

This product has been referenced in:
  • Li J  et al. Nuclear PKC-? facilitates rapid transcriptional responses in human memory CD4+ T cells through p65 and H2B phosphorylation. J Cell Sci 129:2448-61 (2016). WB ; Human . Read more (PubMed: 27149922) »
  • Aguiló JI  et al. Protein kinase C-theta is required for NK cell activation and in vivo control of tumor progression. J Immunol 182:1972-81 (2009). WB ; Mouse . Read more (PubMed: 19201850) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Tissue lysate - whole (LIVER)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Dec 06 2016

I did check with the lab regarding the protocol used, the cells should be serum starved before PMA treatment and the concentration is 200nM.