Anti-PKC theta/PRKCQ 抗体 [M217] (ab76197)
Key features and details
- Mouse monoclonal [M217] to PKC theta/PRKCQ
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-PKC theta/PRKCQ antibody [M217]
PKC theta/PRKCQ 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [M217] to PKC theta/PRKCQ -
由来種
Mouse -
特異性
ab76197 is not predicted to react with other PKC family members due to low sequence homology. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: Jurkat whole cell lysate (ab7899). ICC/IF: PC-12 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
M217 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab76197の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100.
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WB |
1/1000. Predicted molecular weight: 82 kDa.
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特記事項 |
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ICC/IF
1/100. |
WB
1/1000. Predicted molecular weight: 82 kDa. |
ターゲット情報
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機能
This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production.
PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. -
組織特異性
Skeletal muscle, megakaryoblastic cells and platelets. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 C2 domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
ドメイン
The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor and the C2 domain is a non-calcium binding domain. -
翻訳後修飾
Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PKC upon antigen receptor ligation. - Information by UniProt
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参照データベース
- Entrez Gene: 5588 Human
- Entrez Gene: 85420 Rat
- Omim: 600448 Human
- SwissProt: Q04759 Human
- SwissProt: Q9WTQ0 Rat
- Unigene: 498570 Human
- Unigene: 225125 Rat
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別名
- KPCT_HUMAN antibody
- MGC126514 antibody
- MGC141919 antibody
see all
画像
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Lane 1 : Anti-PKC theta/PRKCQ antibody [M217] (ab76197) at 1/250 dilution
Lane 2 : Anti-PKC theta/PRKCQ antibody [M217] (ab76197) at 1/1000 dilution
Lane 1 : Jurkat cell lysate.
Lane 2 : Jurkat cell lysate
Predicted band size: 82 kDa
Observed band size: 79 kDa why is the actual band size different from the predicted? -
NGF-differentiated PC-12 cells (rat adrenal gland pheochromocytoma cell line) stained for PKC theta/PRKCQ using ab76197 at 1/100 dilution in ICC/IF. Secondary antibody is a Cy3 conjugate.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab76197 は 1 報の論文で使用されています。
- Zhou LJ et al. Toxoplasma gondii SAG1 targeting host cell S100A6 for parasite invasion and host immunity. iScience 24:103514 (2021). PubMed: 34950858