製品の概要

  • 製品名Anti-PKC nu antibody
    PKC nu 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PKC nu
  • アプリケーション適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to amino acids 367-384 of Human PKC nu.

  • ポジティブ・コントロール
    • PMA-stimulated HL60 cell lysate.
  • 特記事項


    Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度Protein G purified
  • 特記事項(精製)This antibody is purified through a protein G column and eluted out with both high and low pH buffers. It is neutralized immediately after elution, followed by dialysis against PBS.
  • 一次抗体 備考Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The AGC kinase group consists of 63 kinases including the cyclic nucleotide-regulated protein kinase (PKA & PKG) family, the diacylglycerol-activated/phospholipid-dependent protein kinase C (PKC) family, the related to PKA and PKC (RAC/Akt) protein kinase family, the kinases that phosphorylate G protein-coupled receptors family (ARK), and the kinases that phosphorylate ribosomal protein S6 family (RSK). The calcium/calmodulin-dependent kinase (CAMK) group consists of 75 kinases regulated by Ca2+/CaM and close relative family (CAMK, CAMKL, DAPK, MAPKAPK).
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab5285 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 100 kDa.
ELISA 1/1000.

ターゲット情報

  • 機能Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
  • 組織特異性Ubiquitous.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 細胞内局在Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EPK 2 antibody
    • EPK2 antibody
    • KPCD3_HUMAN antibody
    • nPKC nu antibody
    • nPKC-nu antibody
    • nPKCnu antibody
    • nu antibody
    • PKCnu antibody
    • PKD 3 antibody
    • PKD3 antibody
    • PRK D3 antibody
    • PRKCN antibody
    • PRKD 3 antibody
    • Prkd3 antibody
    • Protein kinase C antibody
    • Protein kinase C nu antibody
    • Protein kinase C nu type antibody
    • Protein kinase D3 antibody
    • Protein kinase EPK 2 antibody
    • Protein kinase EPK2 antibody
    • Serine threonine protein kinase antibody
    • Serine/threonine protein kinase D3 antibody
    • Serine/threonine-protein kinase D3 antibody
    see all

Anti-PKC nu antibody 画像

  • Western blot analysis of ab5285 in lysate of HL60 cells stimulated with PMA. PKCnu (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
  • ab5285 staining PKC nu in human breast carcinoma (BC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

Anti-PKC nu antibody (ab5285) 使用論文

ab5285 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"