製品の概要

  • 製品名Anti-PKC mu (phospho Y463) antibody
    PKC mu 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PKC mu (phospho Y463)
  • 特異性Detects endogenous levels of PKC µ only when phosphorylated at tyrosine 463 (corresponding to mouse tyrosine 469 or rat tyrosine 200).
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic phosphopeptide derived from human PKC µ around the phosphorylation site of tyrosine 463 (R-Y-YP-K-E).

  • ポジティブ・コントロール
    • Brain tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab59415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration.
IHC-P 1/50 - 1/100.
ELISA 1/40000.

ターゲット情報

  • 機能Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 細胞内局在Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KPCD1_HUMAN antibody
    • nPKC D1 antibody
    • nPKC mu antibody
    • nPKC-D1 antibody
    • nPKC-mu antibody
    • nPKCD1 antibody
    • nPKCmu antibody
    • PKC antibody
    • PKC MU antibody
    • PKCM antibody
    • PKCmu antibody
    • PKD 1 antibody
    • PKD antibody
    • PKD1 antibody
    • PRKCM antibody
    • PRKD 1 antibody
    • Prkd1 antibody
    • Protein kinase C mu antibody
    • Protein kinase C mu type antibody
    • Protein kinase D antibody
    • Protein kinase D1 antibody
    • Serine/threonine protein kinase D1 antibody
    • Serine/threonine-protein kinase D1 antibody
    see all

Anti-PKC mu (phospho Y463) antibody 画像

  • Immunohistochemical analysis of paraffin embedded human brain tissue using ab59415 at 1/50 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.

Anti-PKC mu (phospho Y463) antibody (ab59415) 使用論文

This product has been referenced in:
  • Zheng H  et al. Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor. J Biol Chem 286:35733-41 (2011). WB ; Human . Read more (PubMed: 21865166) »
  • Arun SN  et al. Ultraviolet B irradiation and activation of protein kinase D in primary mouse epidermal keratinocytes. Oncogene : (2010). WB ; Mouse . Read more (PubMed: 21132013) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HeLa cells)
Loading amount 35 µg
Specification HeLa cells
Treatment 3000 IU/ml interferon alpha for 10 min
Gel Running Conditions Reduced Denaturing (7.5)
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
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投稿 Sep 30 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"