Anti-PKC mu/PKD (phospho Y463) 抗体 (ab59415)
Key features and details
- Rabbit polyclonal to PKC mu/PKD (phospho Y463)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PKC mu/PKD (phospho Y463) antibody
PKC mu/PKD 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PKC mu/PKD (phospho Y463) -
由来種
Rabbit -
特異性
Detects endogenous levels of PKC µ/PKD only when phosphorylated at tyrosine 463 (corresponding to mouse tyrosine 469 or rat tyrosine 200).
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アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human PKC mu/PKD aa 400-500 (phospho Y463).
Database link: Q15139 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab59415の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use at an assay dependent concentration.
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IHC-P |
1/50 - 1/100.
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特記事項 |
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WB
Use at an assay dependent concentration. |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B. -
配列類似性
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
Contains 1 PH domain.
Contains 2 phorbol-ester/DAG-type zinc fingers.
Contains 1 protein kinase domain. -
細胞内局在
Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation. - Information by UniProt
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参照データベース
- Entrez Gene: 5587 Human
- Omim: 605435 Human
- SwissProt: Q15139 Human
- Unigene: 508999 Human
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別名
- KPCD1_HUMAN antibody
- nPKC D1 antibody
- nPKC mu antibody
see all
画像
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All lanes : Anti-PKC mu/PKD (phospho Y463) antibody (ab59415)
Lane 1 : HepG2 cell lysates
Lane 2 : HepG2 cell lysates with Phospho peptide -
Immunohistochemical analysis of paraffin embedded human brain tissue using ab59415 at 1/50 dilution, in the presence (right) or absence (left) of immunising phosphopeptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab59415 は 3 報の論文で使用されています。
- Clift D et al. A Method for the Acute and Rapid Degradation of Endogenous Proteins. Cell 171:1692-1706.e18 (2017). PubMed: 29153837
- Zheng H et al. Tyrosine phosphorylation of protein kinase D2 mediates ligand-inducible elimination of the Type 1 interferon receptor. J Biol Chem 286:35733-41 (2011). WB ; Human . PubMed: 21865166
- Arun SN et al. Ultraviolet B irradiation and activation of protein kinase D in primary mouse epidermal keratinocytes. Oncogene : (2010). WB ; Mouse . PubMed: 21132013