製品の概要

  • 製品名Anti-PKC mu (phospho S916) antibody [EP1494Y]
    PKC mu 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1494Y] to PKC mu (phospho S916)
  • 特異性This antibody detects PKC mu phosphorylated on Serine 916.
  • アプリケーション適用あり: WB, IHC-P, IPmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic phosphopeptide corresponding to residues surrounding S916 of Human PKC mu

  • ポジティブ・コントロール
    • WB: lysate from HeLa cells treated with TPA. IHC: Human placenta tissue.
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81218 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000 - 1/100000. Predicted molecular weight: 102 kDa.
IHC-P 1/100 - 1/250.
IP 1/60.
  • 追加情報Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
    • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
      Contains 1 PH domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • 細胞内局在Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
    • Information by UniProt
    • 参照データベース
    • 別名
      • KPCD1_HUMAN antibody
      • nPKC D1 antibody
      • nPKC mu antibody
      • nPKC-D1 antibody
      • nPKC-mu antibody
      • nPKCD1 antibody
      • nPKCmu antibody
      • PKC antibody
      • PKC MU antibody
      • PKCM antibody
      • PKCmu antibody
      • PKD 1 antibody
      • PKD antibody
      • PKD1 antibody
      • PRKCM antibody
      • PRKD 1 antibody
      • Prkd1 antibody
      • Protein kinase C mu antibody
      • Protein kinase C mu type antibody
      • Protein kinase D antibody
      • Protein kinase D1 antibody
      • Serine/threonine protein kinase D1 antibody
      • Serine/threonine-protein kinase D1 antibody
      see all

    Anti-PKC mu (phospho S916) antibody [EP1494Y] 画像


    • Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 102 kDa


      Exposure time : 12 minutes

      Serum starved HeLa cells were incubated at 37°C for 60 minutes with vehicle control (0 µM) and different concentrations of phorbol 12-myristate 13-acetate (PMA) (ab120297) in DMSO. Increased expression of PKC mu (phospho S916) (ab81218) correlates with an increase in phorbol 12-myristate 13-acetate (PMA) concentration, as described in literature.

      Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 20µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 3% milk before being incubated with ab81218 at 1 µg/ml and ab8227 at 1 µg /ml overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody (ab97051) at 1/10000 dilution and visualised using ECL development solution.

    • All lanes : Anti-PKC mu (phospho S916) antibody [EP1494Y] (ab81218) at 1/100000 dilution

      Lane 1 : lysate from untreated HeLa cells
      Lane 2 : lyaste from HeLa cells treated with TPA

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti-Rabbit at 1/2000 dilution

      Predicted band size : 102 kDa
    • Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using 1/100 ab81218.

    Anti-PKC mu (phospho S916) antibody [EP1494Y] (ab81218) 使用論文

    This product has been referenced in:
    • Harikumar KB  et al. A novel small-molecule inhibitor of protein kinase D blocks pancreatic cancer growth in vitro and in vivo. Mol Cancer Ther 9:1136-46 (2010). Read more (PubMed: 20442301) »

    See 1 Publication for this product

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