製品の概要

  • 製品名
    Anti-PKC mu (phospho S738 + S742) antibody
    PKC mu 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PKC mu (phospho S738 + S742)
  • 特異性
    ab74105 detects endogenous levels of PKC mu only when phosphorylated at serine 738 and serine 742.
  • アプリケーション
    適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthesized phosphopeptide derived from human PKC mu around the phosphorylation site of serine 738 and serine 742 (E-K-SP-F-R-R-SP-V-V).

  • ポジティブ・コントロール
    • WB: extracts from A549 cells treated with PMA (125ng/ml, 30mins), and HT-29 cells treated with serum (20%, 15mins). IHC-P: human heart tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74105 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 102 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
  • 配列類似性
    Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KPCD1_HUMAN antibody
    • nPKC D1 antibody
    • nPKC mu antibody
    • nPKC-D1 antibody
    • nPKC-mu antibody
    • nPKCD1 antibody
    • nPKCmu antibody
    • PKC antibody
    • PKC MU antibody
    • PKCM antibody
    • PKCmu antibody
    • PKD 1 antibody
    • PKD antibody
    • PKD1 antibody
    • PRKCM antibody
    • PRKD 1 antibody
    • Prkd1 antibody
    • Protein kinase C mu antibody
    • Protein kinase C mu type antibody
    • Protein kinase D antibody
    • Protein kinase D1 antibody
    • Serine/threonine protein kinase D1 antibody
    • Serine/threonine-protein kinase D1 antibody
    see all

画像

  • All lanes : Anti-PKC mu (phospho S738 + S742) antibody (ab74105) at 1/500 dilution

    Lane 1 : Extracts from A549 cells treated with PMA (125ng/ml, 30mins), plus phosphopeptide at 10 µg
    Lane 2 : Extracts from HT-29 cells treated with serum (20%, 15mins), plus phosphopeptide at 10 µg
    Lane 3 : Extracts from A549 cells treated with PMA (125ng/ml, 30mins), plus serum (20%, 15mins) and phopeptide at 10 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 102 kDa
    Observed band size : 102 kDa
  • ab74105, at a 1/50 dilution, staining PKC mu in paraffin embedded human heart tissue by Immunohistochemistry in the absence (left image) or presence (right image) of the immunising peptide.

参考文献

This product has been referenced in:
  • Pearen MA  et al. The nuclear receptor, Nor-1, markedly increases type II oxidative muscle fibers and resistance to fatigue. Mol Endocrinol 26:372-84 (2012). WB ; Mouse . Read more (PubMed: 22282471) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Rat Cell lysate - whole cell (Astrocytes)
Specification
Astrocytes
Treatment
10 nM ofControl, Insulin, IGF-1 or both hormones for 15 minutes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Edwin Hernandez

Verified customer

投稿 May 30 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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