製品の概要

  • 製品名Anti-PKC mu antibody
    PKC mu 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to PKC mu
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Drosophila melanogaster
  • 免疫原

    Fusion protein:

    QAGAKERDNFFREEFDDSDGDDGSEYGKYKSAMSSGVNLVAGRPREAPKA LTNAHNSPPELVNGGLVDDSSVSGGETSKSSSDGQSEQSQSSTSSSPSAN

    , corresponding to amino acids 284/383 of Fruit fly (Drosophila melanogaster) PKD

  • 特記事項

    Produced from outbred CD1 mice

     

    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed: 12910245; Barry and Johnston PubMed: 9234514). The animal`s cells produce the protein, which stimulates the animal`s immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 93 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress through activation of NF-kappa-B.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PKD subfamily.
    Contains 1 PH domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 細胞内局在Cytoplasm. Membrane. Translocation to the cell membrane is required for kinase activation.
  • Information by UniProt
  • 別名
    • KPCD1_HUMAN antibody
    • nPKC D1 antibody
    • nPKC mu antibody
    • nPKC-D1 antibody
    • nPKC-mu antibody
    • nPKCD1 antibody
    • nPKCmu antibody
    • PKC antibody
    • PKC MU antibody
    • PKCM antibody
    • PKCmu antibody
    • PKD 1 antibody
    • PKD antibody
    • PKD1 antibody
    • PRKCM antibody
    • PRKD 1 antibody
    • Prkd1 antibody
    • Protein kinase C mu antibody
    • Protein kinase C mu type antibody
    • Protein kinase D antibody
    • Protein kinase D1 antibody
    • Serine/threonine protein kinase D1 antibody
    • Serine/threonine-protein kinase D1 antibody
    see all

Anti-PKC mu antibody 画像

  • All lanes : Anti-PKC mu antibody (ab21938) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a negative control fusion protein with an irrelevant antigen at 20 ug
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen fusion protein at 20 ug

    Secondary
    Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 93 kDa

Anti-PKC mu antibody (ab21938) 使用論文

ab21938 has not yet been referenced specifically in any publications.

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