Anti-PKC delta (phospho Y52) 抗体 (ab74007)

製品の概要

  • 製品名
    Anti-PKC delta (phospho Y52) antibody
    PKC delta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PKC delta (phospho Y52)
  • 特異性
    Detects PKC delta only when phosphorylated at tyrosine 52.
  • アプリケーション
    適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic phosphopeptide derived from human PKC delta around the phosphorylation site of tyrosine 52 (T-M-YP-P-E)

  • ポジティブ・コントロール
    • Human lung carcinoma tissue. Extracts from Jurkat cells. NIH/3T3 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 77 kDa.
ELISA 1/5000.
IHC-P 1/50 - 1/100.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能
    This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
  • 配列類似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • ドメイン
    The C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
    The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.
  • 翻訳後修飾
    Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.
  • 細胞内局在
    Cytoplasm. Membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CVID9 antibody
    • D14Ertd420e antibody
    • Kinase PKC delta antibody
    • KPCD antibody
    • KPCD_HUMAN antibody
    • MAY 1 antibody
    • MAY1 antibody
    • MGC49908 antibody
    • nPKC delta antibody
    • nPKC-delta antibody
    • PCKd antibody
    • PKC d antibody
    • PKC delta antibody
    • PKCD antibody
    • PKCdelta antibody
    • PRKC D antibody
    • PRKC delta antibody
    • Prkcd antibody
    • Protein Kinase C delta antibody
    • Protein kinase C delta type antibody
    • Protein kinase C delta VIII antibody
    • Protein Kinase Cdelta antibody
    • Tyrosine protein kinase PRKCD antibody
    see all

画像

  • Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using ab74007, at 1/50 dilution, in the presence (right panel) or absence (left panel) of the immunising phophopeptide.
  • All lanes : Anti-PKC delta (phospho Y52) antibody (ab74007) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells starved for 24hours
    Lane 2 : Extracts from Jurkat cells starved for 24hours with immunising phosphopeptide at 10 µg

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 77 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 115 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of NIH/3T3 cells using ab74007, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising phosphopeptide.

参考文献

ab74007 has not yet been referenced specifically in any publications.

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