Anti-PKC delta (phospho Y311) 抗体 [EPR2609Y] (ab76181)

製品の概要

  • 製品名Anti-PKC delta (phospho Y311) antibody [EPR2609Y]
    PKC delta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2609Y] to PKC delta (phospho Y311)
  • 特異性ab76181 only detects PKC delta phosphorylated on Tyrosine 311.
  • アプリケーション適用あり: Dot blot, WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC/IF
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A synthetic phospho-peptide from residues surrounding Tyrosine 311 of human PKC delta.

  • ポジティブ・コントロール
    • U937 cell lysate untreated or treated with TPA, human urinary bladder carcinoma tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76181 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Dot blot 1/1000.
WB 1/2500 - 1/5000. Predicted molecular weight: 77 kDa.
IP 1/40.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報Is unsuitable for Flow Cyt or ICC/IF.
  • ターゲット情報

    • 機能This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
    • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • ドメインThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
      The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.
    • 翻訳後修飾Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.
    • 細胞内局在Cytoplasm. Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CVID9 antibody
      • D14Ertd420e antibody
      • Kinase PKC delta antibody
      • KPCD antibody
      • KPCD_HUMAN antibody
      • MAY 1 antibody
      • MAY1 antibody
      • MGC49908 antibody
      • nPKC delta antibody
      • nPKC-delta antibody
      • PCKd antibody
      • PKC d antibody
      • PKC delta antibody
      • PKCD antibody
      • PKCdelta antibody
      • PRKC D antibody
      • PRKC delta antibody
      • Prkcd antibody
      • Protein Kinase C delta antibody
      • Protein kinase C delta type antibody
      • Protein kinase C delta VIII antibody
      • Protein Kinase Cdelta antibody
      • Tyrosine protein kinase PRKCD antibody
      see all

    Anti-PKC delta (phospho Y311) antibody [EPR2609Y] 画像

    • All lanes : Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181) at 1/500 dilution

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate at 200nM for 20 minutes. Whole cell lysates
      Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) treated with Phorbol-12-myristate-13-acetate at 200nM for 20 minutes. Whole cell lysates. Then the membrane was incubated with phosphatase.

      Lysates/proteins at 15 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 77 kDa
      Observed band size : 78 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    • Immunohistochemical analysis of paraffin-embedded human urinary bladder carcinoma with ab76181 at 1/100 dilution.
    • All lanes : Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181) at 1/5000 dilution

      Lane 1 : U937 cell lysate
      Lane 2 : U937 cell lysate treated with TPA

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-rabbit-HRP at 1/2000 dilution

      Predicted band size : 77 kDa
      Observed band size : 78 kDa (why is the actual band size different from the predicted?)
    • Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181) at 1/2500 dilution + C57BL/6 Mouse splenocytes whole cell lysate at 40 µg

      Secondary
      Goat Anti-rabbit IgG (H&L) HRP conjugated polyclonal antibody at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 77 kDa
      Observed band size : 77 kDa


      Exposure time : 10 seconds

      This image is courtesy of an abreview submitted by Bryan Niedenberger Tomar Ghansah

      Blocking- 5% Milk for 1 hour at 25°C.

      A non-specific band was observed at 80 kDa.  

      See Abreview

    • Dot blot analysis of PKC delta (pY311) phospho peptide (Lane 1) and PKC delta non-phospho peptide (Lane 2) using ab76181 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution.

      Blocking and Diluting buffer and concentration: 5% NFDM /TBST.

      Exposure time: 3 minutes.

    Anti-PKC delta (phospho Y311) antibody [EPR2609Y] (ab76181) 使用論文

    This product has been referenced in:
    • Ficarro SB  et al. Online nanoflow multidimensional fractionation for high efficiency phosphopeptide analysis. Mol Cell Proteomics 10:O111.011064 (2011). WB ; Human . Read more (PubMed: 21788404) »

    See 1 Publication for this product

    Product Wall

    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa Cervical Adenocarcinoma, Epithelial)
    Loading amount 40 µg
    Specification HeLa Cervical Adenocarcinoma, Epithelial
    Treatment 100nM Insulin
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Tomar Ghansah

    Verified customer

    投稿 Feb 20 2013

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (C57BL/6 Mouse splenocytes)
    Loading amount 40 µg
    Specification C57BL/6 Mouse splenocytes
    Gel Running Conditions Reduced Denaturing (10% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Tomar Ghansah

    Verified customer

    投稿 Oct 09 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"