Anti-PKC delta (phospho S645) 抗体 [EP1486Y] (ab108972)

製品の概要

  • 製品名Anti-PKC delta (phospho S645) antibody [EP1486Y]
    PKC delta 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1486Y] to PKC delta (phospho S645)
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human PKC delta aa 600 to the C-terminus (phospho S645).
    Database link: Q05655

  • ポジティブ・コントロール
    • 293T cell lysate; Human breat carcinoma; HeLa cells
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108972 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Predicted molecular weight: 78 kDa.
IHC-P 1/50 - 1/100. Antigen retrieval is recommended.
ICC/IF 1/50 - 1/100.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能This is calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters. May play a role in antigen-dependent control of B-cell function. Phosphorylates MUC1 in the C-terminal and regulates the interaction between MUC1 and beta-catenin.
    • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • ドメインThe C1 domain, containing the phorbol ester/DAG-type region 1 (C1A) and 2 (C1B), is the diacylglycerol sensor.
      The C2 domain is a non-calcium binding domain. It binds proteins containing phosphotyrosine in a sequence-specific manner.
    • 翻訳後修飾Phosphorylated on Thr-507, within the activation loop. Autophosphorylated and/or phosphorylated. Although the Thr-507 phosphorylation occurs it is not a prerequisite for enzymatic activity.
    • 細胞内局在Cytoplasm. Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • CVID9 antibody
      • D14Ertd420e antibody
      • Kinase PKC delta antibody
      • KPCD antibody
      • KPCD_HUMAN antibody
      • MAY 1 antibody
      • MAY1 antibody
      • MGC49908 antibody
      • nPKC delta antibody
      • nPKC-delta antibody
      • PCKd antibody
      • PKC d antibody
      • PKC delta antibody
      • PKCD antibody
      • PKCdelta antibody
      • PRKC D antibody
      • PRKC delta antibody
      • Prkcd antibody
      • Protein Kinase C delta antibody
      • Protein kinase C delta type antibody
      • Protein kinase C delta VIII antibody
      • Protein Kinase Cdelta antibody
      • Tyrosine protein kinase PRKCD antibody
      see all

    Anti-PKC delta (phospho S645) antibody [EP1486Y] 画像



    • Predicted band size : 78 kDa

      Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)
      Lanes 2, 6 and 10: PKC delta knockout HAP1 cell lysate (20 µg)
      Lanes 3, 7 and 11: A431 cell lysate (20 µg)
      Lanes 4, 8 and 12: HeLa cell lysate (20 µg)
      Lanes 1, 2, 3 and 4: Green signal from target – ab108972 observed at 78 kDa
      Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa
      Lanes 9, 10, 11 and 12: Merged (red and green) signal

      ab108972 was shown to specifically react with PKC delta when PKC delta knockout samples were used. Wild-type and PKC delta knockout samples were subjected to SDS-PAGE. ab108972 and ab8245 (loading control to GAPDH) were diluted 1/10000 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • Immunofluorescent staining of PKC delta in HeLa cells, using ab108972 at a 1/50 dilution.
    • Immunohistochemical analysis of PKC delta in paraffin embedded Human breast carcinoma tissue, using ab108972 at a 1/50 dilution.


    • Predicted band size : 78 kDa

      Primary ab Dilution 1:100,000 dilution, Secondary ab description and code (ab id) Goat Anti-Rabbit IgG, (H+L), HRP conjugated (ab97051), Secondary ab dilution 1:20,000 dilution, Blocking buffer and concentration 5% NFDM/TBST, Diluting buffer and concentration 5% NFDM/TBST, Lane 1: THP-1 whole cell lysate treated with Calyculin A with no peptides, Lane 2: THP-1 whole cell lysate treated with Calyculin A with PKCd S645Pho peptides, Lane 3: THP-1 whole cell lysate treated with Calyculin A with PKCd unmodified peptides, Lane 4: None, Observed MW   78 kDa, Exposure time   10 seconds



    • Predicted band size : 78 kDa

      Primary ab Dilution 1:100,000 dilution, Secondary ab description and code (ab id) Goat Anti-Rabbit IgG, (H+L), HRP conjugated (ab97051), Secondary ab dilution 1: 20,000 dilution, Blocking buffer and concentration 5% NFDM/TBST, Diluting buffer and concentration 5% NFDM/TBST, Lane 1: Untreated THP-1 (Human monocytic leukemia cell line) whole cell lysates 15ug, Lane 2: THP-1 (Human monocytic leukemia cell line) treated with 50nM Calyculin A for 60 minutes whole cell lysates 15ug. Lane 3: THP-1 (Human monocytic leukemia cell line) treated with 50nM Calyculin A for 60 minutes’ whole cell lysates 15ug. Then the membrane was incubated with phosphatase. Lane 4: None, Observed MW 78 kDa, Exposure time 5 seconds

    • Primary ab dilution 1: 1000 dilution (2.355 μg /ml), Secondary ab description and code (ab id) Goat Anti-Rabbit IgG, (H+L), HRP conjugated (ab97051), Secondary ab dilution    1:100,000 dilution, Blocking buffer and concentration 5% NFDM/TBST, Diluting buffer and concentration 5% NFDM /TBST, Lane 1: PKCd S645Pho peptides, Lane 2: PKCd unmodified peptides, Lane 3: None, Lane 4: None, Observed MW   N/A, Exposure time   3 minutes

    • Antigen PKCd S645Pho peptide, PKCd S645Pho unmodified peptide, Antigen concentration 1000ng/ml, Primary antibody concentration range 0~1000ng/ml, Secondary antibody Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Secondary antibody concentration 1:2500 dilution

    Anti-PKC delta (phospho S645) antibody [EP1486Y] (ab108972) 使用論文

    ab108972 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization Yes - 0.5% Triton X100 in PBS
    Fixative Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Jan 23 2014

    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa Cervical Adenocarcinoma, Epithelial)
    Loading amount 40 µg
    Specification HeLa Cervical Adenocarcinoma, Epithelial
    Gel Running Conditions Reduced Denaturing (12%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Dr. Tomar Ghansah

    Verified customer

    投稿 Feb 12 2013

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"