製品の概要

  • 製品名Anti-PKC Consensus antibody
  • 製品の詳細
    Rabbit polyclonal to PKC Consensus
  • アプリケーション適用あり: ELISAmore details
  • 種交差性
    交差種: Rat, Cow, Human
  • 免疫原

    Synthetic peptide conjugated to Bovine thyroglobulin, corresponding to amino acids 543-550 of PKC Consensus

製品の特性

  • 製品の状態Lyophilised:Reconstitute with 0.1 ml of 10 mg/ml BSA in PBS.
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: Whole serum
  • Concentration information loading...
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab40652 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
  • 追加情報ELISA: Use at an assay dependent dilution.

    With this antibody, we have found that blocking with 5% goat or donkey serum significantly reduces background as compared to BSA or milk.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性Serine/threonine protein kinases phosphorylate the OH group of serine or threonine (which have similar sidechains). Protein kinase C is actually a family of protein kinases consisting of ~10 isozymes. They are divided into three subfamilies: conventional (or classical), novel, and atypical based on their second messenger requirements. While serine/threonine kinases all phosphorylate serine or threonine residues in their substrates, they select specific residues to phosphorylate on the basis of residues that flank the phosphoacceptor site, which together comprise the consensus sequence. Since the consensus sequence residues of the substrate to be phosphorylated make contact with the catalytic cleft of the kinase at several key amino acids (usually through hydrophobic forces and ionic bonds), a kinase is usually not specific to a single substrate, but instead can phosphorylate a whole "substrate family" having common recognition sequences. The consensus sequence of protein kinase C enzymes is similar to that of protein kinase A, since it contains basic amino acids close to the Ser/Thr to be phosphorylated. Their substrates are MARCKS proteins, MAP kinase, transcription factor inhibitor IkappaB, the vitamin D3 receptor VDR, Raf kinase, calpain, and the epidermal growth factor receptor.
    • 細胞内局在Cytoplasmic and Plasma membrane
    • 参照データベース
    • 別名
      • PKC A antibody
      • PKCA antibody
      • PRKCA antibody

    Anti-PKC Consensus antibody (ab40652) 使用論文

    ab40652 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab40652.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"