製品の概要

  • 製品名Anti-PKC beta 2 antibody
    PKC beta 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PKC beta 2
  • アプリケーション適用あり: ELISA, ICC, IHC-Fr, WBmore details
  • 種交差性
    交差種: Rat
    交差が予測される動物種: Mouse, Human, all Mammals
  • 免疫原

    Synthetic peptide:

    SFVNSEFLKPEVKS

    , corresponding to amino acids 660-673 of Rat PKC beta 2.

  • ポジティブ・コントロール
    • Brain and spleen.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.05% Sodium Azide
  • 精製度Whole antiserum
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab4138 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
ICC
IHC-Fr
WB
  • 追加情報ELISA: assay dependent.
    Immunocytochemistry (frozen): 1/250 (using alkaline phosphatase or horseradish peroxidase).
    Western blot (enhanced chemiluminescence): 1/250 (using alkaline phosphatase or horseradish peroxidase). Predicted molecular weight: 80 kDa.
    Not tested in other applications.

    Optimal dilutions / concentrations should be determined by the end user.

    With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.
  • ターゲット情報

    • 機能Calcium-activated and phospholipid-dependent serine/threonine-protein kinase involved in various processes such as regulation of the B-cell receptor (BCR) signalosome, apoptosis and transcription regulation. Plays a key role in B-cell activation and function by regulating BCR-induced NF-kappa-B activation and B-cell suvival. Required for recruitment and activation of the IKK kinase to lipid rafts and mediates phosphorylation of CARD11/CARMA1 at 'Ser-559', 'Ser-644' and 'Ser-652', leading to activate the NF-kappa-B signaling. Involved in apoptosis following oxidative damage: in case of oxidative conditions, specifically phosphorylates 'Ser-36' of isoform p66Shc of SHC1, leading to mitochondrial accumulation of p66Shc, where p66Shc acts as a reactive oxygen species producer. Acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and specifically mediating phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag for epigenetic transcriptional activation that prevents demethylation of histone H3 'Lys-4' (H3K4me) by LSD1/KDM1A. Also involved in triglyceride homeostasis. Serves as the receptor for phorbol esters, a class of tumor promoters.
    • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
      Contains 1 AGC-kinase C-terminal domain.
      Contains 1 C2 domain.
      Contains 2 phorbol-ester/DAG-type zinc fingers.
      Contains 1 protein kinase domain.
    • 翻訳後修飾Phosphorylation on Thr-500 within the activation loop renders it competent to autophosphorylate. Subsequent autophosphorylation of Thr-642 maintains catalytic competence, and autophosphorylation on Ser-661 appears to release the kinase into the cytosol. Autophosphorylation on other sites i.e. in the N-terminal and hinge regions have no effect on enzyme activity.
    • 細胞内局在Cytoplasm. Nucleus. Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • KPCB_HUMAN antibody
      • PKC Beta antibody
      • PKC-B antibody
      • PKC-beta antibody
      • PKCB antibody
      • Prkcb antibody
      • PRKCB II antibody
      • PRKCB2 antibody
      • Protein kinase C beta antibody
      • Protein kinase C beta type antibody
      see all

    Anti-PKC beta 2 antibody (ab4138) 使用論文

    ab4138 has not yet been referenced specifically in any publications.

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    Thank you for getting back to me. Thank you also for trying my suggestions. I am sorry that they did not lead to any improvements to the results that you have been obtaining. As mentioned previously unfortunately we cannot guarantee this antibody to wo...

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    Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. As my colleague has explained unfortunately this antibody is yet to be tested using mouse and human tissue. However, I would predict its react...

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    Thank you for the protocol details that you have provided and I'm sorry to hear that you're experiencing difficulty with ab4138. I noticed that you are using human and mouse samples. Ab4138 has been tested for cross-reactivity with rat PKC beta 2 and a...

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    Thank you for your enquiry and for your detailed protocol. This antibody recognizes Rat PKC beta 2 and the predicted molecular weight is kDa. We would like to emphasize the fact that this antibody has only been tested on rat samples. Since you ar...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"