Anti-PKC alpha (phospho T638) 抗体 [E195] (ab32502)

製品の概要

  • 製品名Anti-PKC alpha (phospho T638) antibody [E195]
    PKC alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [E195] to PKC alpha (phospho T638)
  • 特異性The antibody only detects PKC alpha phosphorylated on Threonine 638.
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IPmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow
  • 免疫原

    A synthetic phospho-peptide corresponding to residues surrounding Thr638 of human PKC alpha.

  • ポジティブ・コントロール
    • 293 cell lysate and normal brain tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/20000. Detects a band of approximately 80 kDa (predicted molecular weight: 77 kDa).
IHC-P 1/250.
ICC/IF 1/500.
IP 1/40.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    Anti-PKC alpha (phospho T638) antibody [E195] 画像

    • All lanes : Anti-PKC alpha (phospho T638) antibody [E195] (ab32502) at 1/20000 dilution

      Lane 1 : 293 cell lysate, untreated
      Lane 2 : 293 cell lysate, treated with TPA.


      Predicted band size : 77 kDa
      Observed band size : 80 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical staining of untreated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution.
    • Immunohistochemical staining of Phosphatase-treated paraffin-embedded normal brain tissue using ab32502 at 1/250 dilution

    Anti-PKC alpha (phospho T638) antibody [E195] (ab32502) 使用論文

    This product has been referenced in:

    See all 5 Publications for this product

    Product Wall

    We have not yet tested this item for cross reactivity against other PKC family members. BLASTs and immunogen alignment that I have performed show that this product, has 85% and 66% homology against mouse PKC beta and gamma respectively. Alignment again...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"