The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 77 kDa).
1/500. Antigen retrieval is not essential but may optimise staining.
Use at an assay dependent concentration. PubMed: 23374330
機能This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.
ab86715, at 1/500 dilution, staining PKC alpha in formalin-fixed, paraffin-embedded, citric acid treated adult mouse brain by Immunohistochemistry, secondary stained with anti-mouse HRP followed by DAB detection.
Anti-PKC alpha antibody [M237] (ab86715) 使用論文
This product has been referenced in:
Huang JF et al. Differential neuronal expression of receptor interacting protein 3 in rat retina: involvement in ischemic stress response. BMC Neurosci14:16 (2013).
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