The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
機能This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 C2 domain. Contains 2 phorbol-ester/DAG-type zinc fingers. Contains 1 protein kinase domain.
Lane 1: ab87920 at 3 µg/mg of lysate was used to immunoprecipitate PKC alpha from 1 mg of HeLa whole cell lysate (20% of IP loaded).
Lane 2: as above, with a control IgG used for immunoprecipitation.
Detection: ab87920 at 1 &micro;g/ml followed by ECL with an exposure time of 3 seconds.
Anti-PKC alpha antibody (ab87920) 使用論文
has not yet been referenced specifically in any publications.