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Our Abpromise guarantee covers the use of ab4124 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/250. Predicted molecular weight: 80 kDa. (AP/HRP). With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: HEK293 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab4124 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab4124 was shown to recognize PKC alpha when PKC alpha knockout samples were used, along with additional cross-reactive bands. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab4124 and ab8245 (loading control to GAPDH) were diluted to 1/250 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.