製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PKC alpha
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Synthetic peptide:

    SYVNPQFVHPILQSAV

    , corresponding to amino acids 657-672 of Rat PKC alpha.

  • ポジティブ・コントロール
    • ICC/IF: MCF7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/250. Predicted molecular weight: 80 kDa. (AP/HRP). With a more sensitive detection system, such as enhanced chemiluminescence or ELISA, the antibody may be diluted further.

ターゲット情報

  • 機能
    This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 配列類似性
    Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 細胞内局在
    Cytoplasm. Cell membrane. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AAG6 antibody
    • Aging associated gene 6 antibody
    • aPKC antibody
    • KPCA_HUMAN antibody
    • PKC alpha antibody
    • PKC-A antibody
    • PKC-alpha antibody
    • PKCA antibody
    • PRKACA antibody
    • PRKCA antibody
    • Protein Kinase C alpha antibody
    • Protein kinase C alpha type antibody
    see all

画像

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab4124 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.


    ab4124 was shown to recognize PKC alpha in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when PKC alpha knockout samples were examined. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab4124 and ab8245 (loading control to GAPDH) were diluted to 1/250 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: PKC alpha knockout HAP1 cell lysate (20 µg)
    Lane 3: K562 cell lysate (20 µg)
    Lane 4: HEK293 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab4124 observed at 77 kDa. Red - loading control, ab8245, observed at 37 kDa.

    This western blot image is a comparison between ab4124 and a competitor's top cited rabbit polyclonal antibody.

プロトコール

参考文献

This product has been referenced in:
  • Li Y  et al. Mechanism of the protective effects of the combined treatment with rhynchophylla total alkaloids and sinapine thiocyanate against a prothrombotic state caused by vascular endothelial cell inflammatory damage. Exp Ther Med 13:3081-3088 (2017). Read more (PubMed: 28587383) »
  • Griffon A  et al. Integrative analysis of public ChIP-seq experiments reveals a complex multi-cell regulatory landscape. Nucleic Acids Res 43:e27 (2015). Read more (PubMed: 25477382) »

See all 7 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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