製品の概要

  • 製品名Anti-PKC alpha antibody [133]
    PKC alpha 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [133] to PKC alpha
  • 特異性ab11723 recognises the alpha isoform of PKC. This antibody recognises the sequence PQFVHPILQSAV at the C terminus of PKC alpha.
  • アプリケーション適用あり: ICC/IF, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat
  • 免疫原

    PKC alpha.

  • 特記事項This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab11723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration. PubMed: 17158168
WB 1/10 - 1/500. Predicted molecular weight: 80.6 kDa.
IHC-Fr 1/1 - 1/10.

ターゲット情報

  • 機能This is a calcium-activated, phospholipid-dependent, serine- and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4.
    PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
  • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 C2 domain.
    Contains 2 phorbol-ester/DAG-type zinc fingers.
    Contains 1 protein kinase domain.
  • 細胞内局在Cytoplasm. Cell membrane. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AAG6 antibody
    • Aging associated gene 6 antibody
    • aPKC antibody
    • KPCA_HUMAN antibody
    • PKC alpha antibody
    • PKC-A antibody
    • PKC-alpha antibody
    • PKCA antibody
    • PRKACA antibody
    • PRKCA antibody
    • Protein Kinase C alpha antibody
    • Protein kinase C alpha type antibody
    see all

Anti-PKC alpha antibody [133] 画像



  • Predicted band size : 80.6 kDa

    Lanes 1, 5 and 9: Wild-type HAP1 cell lysate (20 µg)

    Lanes 2, 6 and 10: PKC alpha knockout HAP1 cell lysate (20 µg)

    Lanes 3, 7 and 11: K562 cell lysate (20 µg)

    Lanes 4, 8 and 12: HEK293 cell lysate (20 µg)

    Lanes 1, 2, 3 and 4: Green signal from target – ab11723 observed at 77 kDa

    Lanes 5, 6, 7 and 8: Red signal from loading control – ab8245 observed at 37 kDa

    Lanes 9, 10, 11 and 12: Merged (red and green) signal

    ab11723 was shown to specifically react with PKC alpha when PKC alpha knockout samples were used. Wild-type and PKC alpha knockout samples were subjected to SDS-PAGE. ab11723 and ab8245 (loading control to GAPDH) were diluted 1/500 and 1/2000 respectively and incubated overnight at 4ºC. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

Anti-PKC alpha antibody [133] (ab11723) 使用論文

This product has been referenced in:
  • Cao Y  et al. Regulators of G protein signaling RGS7 and RGS11 determine the onset of the light response in ON bipolar neurons. Proc Natl Acad Sci U S A 109:7905-10 (2012). Read more (PubMed: 22547806) »
  • Vargas-Medrano J  et al. PKCß-dependent phosphorylation of the glycine transporter 1. Neurochem Int 59:1123-32 (2011). Read more (PubMed: 21864610) »

See all 9 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Specification HeLa cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Dec 05 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"