The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2500. Detects a band of approximately 51 kDa (predicted molecular weight: 45 kDa).
Is unsuitable for Flow Cyt.
Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
Belongs to the cAMP-dependent kinase regulatory chain family. Contains 2 cyclic nucleotide-binding domains.
Phosphorylated by the activated catalytic chain.
Cytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane.
Other comments: Confocal image showed increased cytoplasmic staining after CA (100ng/ml, 10min) treatment on Hela cells. The LP treatment decreased the increased cytoplasmic staining caused by CA. Ab32514 was used as a Pan control for ab32390. The results showed cytoplasmic staining on untreated, CA and CA+LP treated Hela cells.
Western blot - Anti-PKA R2 (phospho S99) antibody [E151] (ab32390)
All lanes : Anti-PKA R2 (phospho S99) antibody [E151] (ab32390) at 1/2000 dilution
Lane 1 : K562 whole cell lysate with PKA R2 (pS99) phospho peptide Lane 2 : K562 whole cell lysate with PKA R2 non-phospho peptide
Secondary All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution