製品の概要

  • 製品名Anti-PKA R2 antibody [Y116]
    PKA R2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y116] to PKA R2
  • 特異性The antibody recognises PKA RII. It does not cross-react with other cAMP-dependent kinase.
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IPmore details
    適用なし: Flow Cyt
  • 種交差性
    交差種: Cow, Human
  • 免疫原

    A synthetic peptide corresponding to residues in the C-terminus of human cAMP-dependent protein kinase type II-alpha regulatory subunit.

  • ポジティブ・コントロール
    • K562 cell lysate, HeLa cells and normal breast tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab32514 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/10000 - 1/50000. Detects a band of approximately 52 kDa (predicted molecular weight: 45 kDa).
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
IP 1/50.
  • 追加情報Is unsuitable for Flow Cyt.
  • ターゲット情報

    • 機能Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
    • 組織特異性Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
    • 配列類似性Belongs to the cAMP-dependent kinase regulatory chain family.
      Contains 2 cyclic nucleotide-binding domains.
    • 翻訳後修飾Phosphorylated by the activated catalytic chain.
    • 細胞内局在Cytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • cAMP dependent protein kinase regulatory subunit alpha 2 antibody
      • cAMP dependent protein kinase regulatory subunit RII alpha antibody
      • cAMP dependent protein kinase type II alpha regulatory chain antibody
      • cAMP dependent protein kinase type II alpha regulatory subunit antibody
      • cAMP-dependent protein kinase type II-alpha regulatory subunit antibody
      • KAP2 antibody
      • KAP2_HUMAN antibody
      • MGC3606 antibody
      • PKR 2 antibody
      • PKR2 antibody
      • PRKA R2 antibody
      • PRKAR 2 antibody
      • PRKAR2 antibody
      • PRKAR2A antibody
      • Protein kinase A RII alpha subunit antibody
      • Protein kinase cAMP dependent regulatory type II alpha antibody
      see all

    Anti-PKA R2 antibody [Y116] 画像

    • Anti-PKA R2 antibody [Y116] (ab32514) at 1/50000 dilution + K562 cell lysate

      Predicted band size : 45 kDa
      Observed band size : 52 kDa (why is the actual band size different from the predicted?)
    • Immunohistochemical analysis of paraffin-embedded human normal breast tissue using ab32514 at 1/100 dilution.
    • Immunofluorescent staining of HeLa cells using ab32514 at 1/100 dilution.

    Anti-PKA R2 antibody [Y116] (ab32514) 使用論文

    This product has been referenced in:
    • Hillman P  et al. Dissociation between AKAP3 and PKARII promotes AKAP3 degradation in sperm capacitation. PLoS One 8:e68873 (2013). WB ; Cow . Read more (PubMed: 23894359) »
    • Gu L  et al. Protein kinase A activation confers resistance to trastuzumab in human breast cancer cell lines. Clin Cancer Res 15:7196-206 (2009). IHC-P ; Human . Read more (PubMed: 19920112) »

    See all 2 Publications for this product

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    There are currently no Abreviews or Questions for ab32514.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"