製品の概要

  • 製品名Anti-PKA R2 antibody
    PKA R2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PKA R2
  • 特異性ab38949 binds to PKARIIa, but does not cross react with the other PKA regulatory subunits (PKARIa, PKARIb, PKARIIb) and can recognize native or reduced PKARIIa.
  • アプリケーション適用あり: WB, ELISA, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Human
  • 免疫原

    Synthetic peptide of Rat cAMP-dependent protein kinase type II-alpha regulatory subunit. (Peptide available as ab41297.)

  • 特記事項Store diluted antibody at 4°C with 0.05% Sodium Azide as preservative.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Detects a band of approximately 55, 53 kDa (predicted molecular weight: 45 kDa).
ELISA Use at an assay dependent dilution.
IP Use at an assay dependent dilution.

ターゲット情報

  • 機能Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
  • 組織特異性Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
  • 配列類似性Belongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.
  • 翻訳後修飾Phosphorylated by the activated catalytic chain.
  • 細胞内局在Cytoplasm. Cell membrane. Colocalizes with PJA2 in the cytoplasm and the cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cAMP dependent protein kinase regulatory subunit alpha 2 antibody
    • cAMP dependent protein kinase regulatory subunit RII alpha antibody
    • cAMP dependent protein kinase type II alpha regulatory chain antibody
    • cAMP dependent protein kinase type II alpha regulatory subunit antibody
    • cAMP-dependent protein kinase type II-alpha regulatory subunit antibody
    • KAP2 antibody
    • KAP2_HUMAN antibody
    • MGC3606 antibody
    • PKR 2 antibody
    • PKR2 antibody
    • PRKA R2 antibody
    • PRKAR 2 antibody
    • PRKAR2 antibody
    • PRKAR2A antibody
    • Protein kinase A RII alpha subunit antibody
    • Protein kinase cAMP dependent regulatory type II alpha antibody
    see all

Anti-PKA R2 antibody 画像

  • ICC/IF image of ab38949 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38949, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ICC/IF image of ab38949 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38949, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

プロトコール

Anti-PKA R2 antibody (ab38949) 使用論文

This product has been referenced in:
  • Beristain AG  et al. PKA signaling drives mammary tumorigenesis through Src. Oncogene N/A:N/A (2014). Human . Read more (PubMed: 24662820) »
  • Nichols CB  et al. ß-adrenergic signaling inhibits Gq-dependent protein kinase D activation by preventing protein kinase D translocation. Circ Res 114:1398-409 (2014). WB . Read more (PubMed: 24643961) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Treatment 20 ug/ml poly(I:C)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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Abcam user community

Verified customer

投稿 Jul 03 2014

Thank you for your reply. The only information that we have on the specificity of the antibody is that which is listed on the datasheet, so there is no additional data that I can provide you with unfortunately. If you are going to be using the antibody...

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Thank you for contacting Abcam. The immunogen sequence for ab38949 is proprietary and I don't have access to that information. It does say on the datasheet that the antibody will not cross react with the other PKA regulatory subunits (PKARIa, PKARIb, P...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"