Anti-PKA beta (catalytic subunit) 抗体 (ab94612)

製品の概要

  • 製品名Anti-PKA beta (catalytic subunit) antibody
    PKA beta (catalytic subunit) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PKA beta (catalytic subunit)
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Caenorhabditis elegans, Drosophila melanogaster, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within the internal sequence amino acids 288-337 (GVSDIKTHKW FATTDWIAIY QRKVEAPFIP KFRGSGDTSN FDDYEEEDIR) of Human PKA beta (catalytic subunit)(NP_002722).

  • ポジティブ・コントロール
    • Human fetal lung lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab94612 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.
  • 組織特異性Isoform 1 is most abundant in the brain, with low level expression in kidney. Isoform 2 is predominantly expressed in thymus, spleen and kidney. Isoform 3 and isoform 4 are only expressed in the brain.
  • 配列類似性Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily.
    Contains 1 AGC-kinase C-terminal domain.
    Contains 1 protein kinase domain.
  • 翻訳後修飾Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively.
  • 細胞内局在Cytoplasm. Nucleus. Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • cAMP-dependent protein kinase catalytic beta subunit isoform 4ab antibody
    • cAMP-dependent protein kinase catalytic subunit beta antibody
    • KAPCB_HUMAN antibody
    • PKA C beta antibody
    • PKA C-beta antibody
    • PKACB antibody
    • Prkacb antibody
    • protein kinase A catalytic subunit beta antibody
    • Protein kinase cAMP dependent catalytic beta antibody
    see all

Anti-PKA beta (catalytic subunit) antibody 画像

  • Anti-PKA beta (catalytic subunit) antibody (ab94612) at 1 µg/ml (in 5% skim milk / PBS buffer) + Human fetal lung lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 41 kDa
  • All lanes : Anti-PKA beta (catalytic subunit) antibody (ab94612) at 1 µg/ml

    Lane 1 : Recombinant human PKA beta (catalytic subunit) protein (ab60837) at 0.1 µg
    Lane 2 : Recombinant human PKA beta (catalytic subunit) protein (ab60837) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Anti-PKA beta (catalytic subunit) antibody (ab94612) 使用論文

ab94612 has not yet been referenced specifically in any publications.

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