Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) 抗体 [EP2606Y] (ab75991)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP2606Y] to PKA alpha/beta/gamma (catalytic subunit) (phospho T197)
- Suitable for: WB, IHC-P, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y]
PKA alpha/beta/gamma (catalytic subunit) 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP2606Y] to PKA alpha/beta/gamma (catalytic subunit) (phospho T197) -
由来種
Rabbit -
特異性
Detects PKA alpha/beta/gamma (catalytic subunit) phosphorylated on threonine 197. Moreover, the antibody will cross-react with PRKX/Y (pT203). -
アプリケーション
適用あり: WB, IHC-P, Dot blotmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human PKA alpha/beta/gamma (catalytic subunit) aa 150-250 (phospho T197). The exact sequence is proprietary.
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ポジティブ・コントロール
- WB: HeLa, NIH/3T3, C6 whole cell lysate IHC: human liver carcinoma, mouse kidney, rat kidney
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特記事項
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP2606Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab75991の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
1/1000. Predicted molecular weight: 40 kDa.
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IHC-P |
1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use of HRP-conjugated or polymerized HRP secondary antibodies recommended, stronger signals have been found using the polymerized HRP secondary. |
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Dot blot |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/1000. Predicted molecular weight: 40 kDa. |
IHC-P
1/5000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP-conjugated or polymerized HRP secondary antibodies recommended, stronger signals have been found using the polymerized HRP secondary. |
Dot blot
Use at an assay dependent concentration. |
ターゲット情報
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参照データベース
- Entrez Gene: 5566 Human
- Entrez Gene: 5567 Human
- Entrez Gene: 5568 Human
- Entrez Gene: 18747 Mouse
- Entrez Gene: 18749 Mouse
- Entrez Gene: 25636 Rat
- Entrez Gene: 293508 Rat
- Omim: 176892 Human
see all -
別名
- cAMP dependent protein kinase catalytic subunit alpha antibody
- cAMP dependent protein kinase catalytic subunit beta antibody
- cAMP dependent protein kinase catalytic subunit gamma antibody
see all
画像
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All lanes : Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) at 1/1000 dilution (Purified)
Lane 1 : Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes, whole cell lysate
Lane 3 : HeLa (Human cervix adenocarcinoma epithelial cell) starved overnight, then treated with 100nM Calyculin A for 30 minutes whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa -
All lanes : Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour
Lane 3 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 4 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour
Lane 5 : C6 (Rat glial tumor glial cell) whole cell lysate
Lane 6 : C6 (Rat glial tumor glial cell) whole cell lysate, then the membrane treated with Lambda Phosphatase for 1 hour
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue sections labeling PKA alpha/beta/gamma (catalytic subunit) with Purified ab75991 at 1/5000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling PKA alpha/beta/gamma (catalytic subunit) with Purified ab75991 at 1/5000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver carcinoma tissue sections labeling PKA alpha/beta/gamma (catalytic subunit) with Purified ab75991 at 1/5000 dilution (0.05 µg/mL). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used at 1/0 dilution. PBS instead of the primary antibody was used as the negative control. M3
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All lanes : Anti-PKA alpha/beta/gamma (catalytic subunit) (phospho T197) antibody [EP2606Y] (ab75991) at 1/1000 dilution (Unpurified)
Lane 1 : Jurkat (Human T cell leukemia T lymphocyte) 10% FBS all the time. Whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia T lymphocyte) serum starve for 24h then 20% FBS 20h. Whole cell lysates
Lane 3 : Jurkat (Human T cell leukemia T lymphocyte) serum starve for 24h then 20% FBS 20h.Whole cell lysates.Then the membrane was incubated with phosphatase.
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Exposure time: 1 secondBlocking and diluting buffer and concentration: 5% NFDM/TBST.
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Dot blot analysis of various peptides labelling PKA gamma (catalytic subunit) (phospho T197) with unpurified ab75991 at a dilution of 1/1000. ab97051, a peroxidase-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/100000).
Lane 1 - PKA alpha/beta/gamma (catalytic subunit) (pT197) phospho peptide.
Lane 2 - PKA alpha/beta/gamma (catalytic subunit) non-phospho peptide.
Lane 3 - PRKX/Y (pT203) phospho peptide.
Lane 4 - PRKX/Y non-phospho peptide.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (50)
ab75991 は 50 報の論文で使用されています。
- Yuan F et al. Effect of phosphodiesterase-4 inhibitor rolipram on colonic hypermotility in water avoidance stress rat model. Neurogastroenterol Motil 34:e14317 (2022). PubMed: 35037375
- Zhan Y et al. Effects of Maren Pills on the Intestinal Microflora and Short-Chain Fatty Acid Profile in Drug-Induced Slow Transit Constipation Model Rats. Front Pharmacol 13:804723 (2022). PubMed: 35496291
- Tie P et al. SLC18A3 promoted renal cancer development through acetylcholine/cAMP signaling. Am J Cancer Res 12:4279-4289 (2022). PubMed: 36225635
- Tao J et al. Sfrp1 protects against acute myocardial ischemia (AMI) injury in aged mice by inhibiting the Wnt/ß-catenin signaling pathway. J Cardiothorac Surg 16:12 (2021). PubMed: 33468190
- Wang J et al. H2 S catalysed by CBS regulates testosterone synthesis through affecting the sulfhydrylation of PDE. J Cell Mol Med 25:3460-3468 (2021). PubMed: 33713531