Anti-PKA 2 beta (regulatory subunit) 抗体 [EP2649Y] (ab75993)

製品の概要

  • 製品名Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y]
    PKA 2 beta (regulatory subunit) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2649Y] to PKA 2 beta (regulatory subunit)
  • アプリケーション適用あり: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human PKA 2 beta (regulatory subunit) (C terminal).

  • ポジティブ・コントロール
    • WB: Human brain lysate IHC-P: Human testis tissue
  • 特記事項

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • バッファーPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • 精製度Tissue culture supernatant
  • ポリ/モノモノクローナル
  • クローン名EP2649Y
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab75993 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
IP
IHC-P
Flow Cyt
ICC/IF
  • 追加情報Flow Cyt: 1/80.
    ICC/IF: 1/100 - 1/250.
    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Use of HRP conjugated or polymerized HRP secondary antibodies recommended. Stronger signals have been found using the polymerized HRP secondary.
    IP: 1/60.
    WB: 1/25,000 - 1/50,000. Predicted molecular weight: 46 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
    • 組織特異性Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
    • 配列類似性Belongs to the cAMP-dependent kinase regulatory chain family.
      Contains 2 cyclic nucleotide-binding domains.
    • 翻訳後修飾Phosphorylated by the activated catalytic chain.
    • 細胞内局在Cytoplasm. Cell membrane. Co-localizes with PJA2 in the cytoplasm and at the cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • AI451071 antibody
      • AW061005 antibody
      • cAMP dependent protein kinase type II beta regulatory chain antibody
      • cAMP dependent protein kinase type II beta regulatory subunit antibody
      • cAMP-dependent protein kinase type II-beta regulatory subunit antibody
      • H RG363E19.2 antibody
      • KAP3_HUMAN antibody
      • MGC116401 antibody
      • Pkarb2 antibody
      • PRKAR 2 antibody
      • PRKAR2 antibody
      • PRKAR2B antibody
      • Protein kinase cAMP dependent regulatory type II beta antibody
      • RATDNA antibody
      • RII beta antibody
      • RII(beta) antibody
      • RIIbeta antibody
      • WUGSC:H RG363E19.2 antibody
      see all

    Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] 画像

    • Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993) at 1/50000 dilution + Human brain lysate at 10 µg

      Secondary
      goat anti rabbit HRP at 1/2000 dilution

      Predicted band size : 46 kDa
      Observed band size : 46 kDa
    • ab75993, at a 1/100 dilution, staining PKA 2 beta (regulatory subunit) in formalin fixed, paraffin embedded human testis tissue by Immunohistochemistry.
    • Overlay histogram showing SH-SY5Y cells stained with ab75993 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab75993, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-PKA 2 beta (regulatory subunit) antibody [EP2649Y] (ab75993) 使用論文

    This product has been referenced in:
    • Nagalla S  et al. Platelet microRNA-mRNA coexpression profiles correlate with platelet reactivity. Blood 117:5189-97 (2011). WB ; Human . Read more (PubMed: 21415270) »

    See 1 Publication for this product

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