Anti-PKA 2 beta (regulatory subunit) 抗体 (ab38222)

製品の概要

  • 製品名
    Anti-PKA 2 beta (regulatory subunit) antibody
    PKA 2 beta (regulatory subunit) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PKA 2 beta (regulatory subunit)
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide selected from the sequence of human PKA 2 beta within aa 1-100, (N terminal region).

  • ポジティブ・コントロール
    • Mouse brain tissue lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab38222 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/500. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ELISA 1/1000.

ターゲット情報

  • 機能
    Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.
  • 組織特異性
    Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.
  • 配列類似性
    Belongs to the cAMP-dependent kinase regulatory chain family.
    Contains 2 cyclic nucleotide-binding domains.
  • 翻訳後修飾
    Phosphorylated by the activated catalytic chain.
  • 細胞内局在
    Cytoplasm. Cell membrane. Co-localizes with PJA2 in the cytoplasm and at the cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AI451071 antibody
    • AW061005 antibody
    • cAMP dependent protein kinase type II beta regulatory chain antibody
    • cAMP dependent protein kinase type II beta regulatory subunit antibody
    • cAMP-dependent protein kinase type II-beta regulatory subunit antibody
    • H RG363E19.2 antibody
    • KAP3_HUMAN antibody
    • MGC116401 antibody
    • Pkarb2 antibody
    • PRKAR 2 antibody
    • PRKAR2 antibody
    • PRKAR2B antibody
    • Protein kinase cAMP dependent regulatory type II beta antibody
    • RATDNA antibody
    • RII beta antibody
    • RII(beta) antibody
    • RIIbeta antibody
    • WUGSC:H RG363E19.2 antibody
    see all

画像

  • Anti-PKA 2 beta (regulatory subunit) antibody (ab38222) at 1/100 dilution + mouse brain tissue lysate

    Predicted band size : 46 kDa
    Observed band size : 46 kDa

プロトコール

参考文献

ab38222 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse brain)
Loading amount
10 µg
Specification
mouse brain
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 May 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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