製品の概要

  • 製品名Anti-PIWIL4 antibody
    PIWIL4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PIWIL4
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Monkey
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 639-839 of Human PIWIL4 (AAH31060).

  • ポジティブ・コントロール
    • MCF7 and IMR32 cells and whole cell lysates; 59T xenograft tissue.

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab111714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 97 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. or Tris-EDTA buffer ( PH8.0)
ICC/IF 1/100 - 1/500.

ターゲット情報

  • 機能Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Associates with secondary piRNAs antisense and PIWIL2/MILI is required for such association. The piRNA process acts upstream of known mediators of DNA methylation. Participates to a piRNA amplification loop. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation (By similarity). May be involved in the chromatin-modifying pathway by inducing 'Lys-9' methylation of histone H3 at some loci.
  • 組織特異性Expressed in testis. According to PubMed:17544373, it is ubiquitously expressed.
  • 配列類似性Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • 翻訳後修飾Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
  • 細胞内局在Nucleus. Cytoplasm. Probable component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. PIWIL2/MILI is required for nuclear localization.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp686P01248 antibody
    • FLJ36156 antibody
    • HILI 2 antibody
    • HILI2 antibody
    • HIWI 2 antibody
    • HIWI2 antibody
    • Miwi 2 protein antibody
    • Miwi2 antibody
    • PIWI antibody
    • Piwi like 2 antibody
    • Piwi like 4 (Drosophila) antibody
    • Piwi like 4 antibody
    • Piwi like protein 4 antibody
    • PIWI like protein antibody
    • Piwi like RNA mediated gene silencing 4 antibody
    • Piwi-like protein 4 antibody
    • PIWIL 4 antibody
    • Piwil4 antibody
    • PIWL4_HUMAN antibody
    see all

Anti-PIWIL4 antibody 画像

  • Anti-PIWIL4 antibody (ab111714) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg

    Predicted band size : 97 kDa
  • ab111714 at 1/200 dilution staining PIWIL4 in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
  • ab111714 at 1/500 dilution staining PIWIL4 in Formalin-fixed, paraffin-embedded 59T xenograft tissue by Immunohistochemistry.

Anti-PIWIL4 antibody (ab111714) 使用論文

ab111714 has not yet been referenced specifically in any publications.

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