製品の概要

  • 製品名Anti-PIWIL1 antibody
    PIWIL1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PIWIL1
  • アプリケーション適用あり: WB, IHC-P, ICC/IF, IPmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from within residues 800 - 900 of Human PIWIL1.

    (Peptide available as ab13827.)

  • ポジティブ・コントロール
    • Miwi is exclusively expressed in spermatocytes and hematopoietic stem cells. WB: Mouse and rat testis tissue lysates. ICC/IF: methanol fixed HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab12337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).Can be blocked with Human PIWIL1 peptide (ab13827).

ab12337 detects a 99 kDa band in both mouse and rat testis lysates (positive controls). Non-specific bands at 102 kDa were also observed in mouse and rat liver lysates (negative controls).

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
IP Use at an assay dependent concentration. PubMed: 19918066

ターゲット情報

  • 機能Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Probable component of some RISC complex, which mediates RNA cleavage and translational silencing. Also plays a role in the formation of chromatoid bodies and is required for some miRNAs stability (By similarity). Isoform 3 may be a negative developmental regulator.
  • 組織特異性Detected in most fetal and adult tissues. Expressed in testes, specifically in germline cells; detected in spermatocytes and spermatids during spermatogenesis. Increased expression in testicular tumors originating from embryonic germ cells with retention of germ cells phenotype. No expression in testicular tumors of somatic origin, such as Sertoli cell and Leydig cell tumors. Overexpressed in gastric cancer cells. Isoform 3 is ubiquitously expressed, and specifically in CD34+ hematopoietic progenitor cells but not in more differentiated cells.
  • 配列類似性Belongs to the argonaute family. Piwi subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • ドメインThe PAZ domain specifically recognizes binds the 2'-O-methylated 3'-end of piRNAs.
  • 翻訳後修飾Arginine methylation by PRMT5 is required for the interaction with Tudor domain-containing protein (TDRD1, TDRKH/TDRD2, RNF17/TDRD4, TDRD6, TDRD7 and TDRD9) and subsequent localization to the meiotic nuage, also named P granule.
  • 細胞内局在Cytoplasm. Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon during meiosis. Also present in chromatoid body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • HIWI antibody
    • MIWI antibody
    • Piwi (Drosophila) like 1 antibody
    • PIWI antibody
    • Piwi homolog antibody
    • Piwi like 1 (drosophila) antibody
    • Piwi like 1 antibody
    • Piwi-like protein 1 antibody
    • PIWIL1 antibody
    • PIWL1_HUMAN antibody
    see all

Anti-PIWIL1 antibody 画像

  • Image courtesy of Human Protein Atlas

    ab12337 staining PIWI Human female colon, showing a distinct and strong staining pattern at glandular cells. Paraffin embedded human colon tissue was incubated with ab12337 (1/200 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6.

    ab12337 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

    .
  • All lanes : Anti-PIWIL1 antibody (ab12337) at 1 µg/ml

    Lane 1 : Testis (Mouse) Tissue Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Testis (Rat) Tissue Lysate - normal tissue (ab29388)
    Lane 4 : Liver (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
    Additional bands at : 102 kDa (possible non-specific binding).

    ab12337 gave a positive signal in both mouse and rat testis lysates (positive controls). Bands were also observed in mouse and rat liver lysates (negative controls) that were higher in size to that observed in testis.

  • ICC/IF image of ab12337 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab12337 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PIWIL1 antibody (ab12337) 使用論文

This product has been referenced in:
  • Xu K  et al. AKAP3 synthesis is mediated by RNA binding proteins and PKA signaling during mouse spermiogenesis. Biol Reprod 90:119 (2014). Read more (PubMed: 24648398) »
  • Castañeda J  et al. Reduced pachytene piRNAs and translation underlie spermiogenic arrest in Maelstrom mutant mice. EMBO J N/A:N/A (2014). Mouse . Read more (PubMed: 25063675) »

See all 8 Publications for this product

Product Wall

Application IHC - Wholemount
Sample Cow Tissue (Testis)
Specification Testis
Username

Dr. Stewart Russell

Verified customer

投稿 Oct 19 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8)
Sample Cow Tissue lysate - whole (Testis, Brain, Ovary)
Specification Testis, Brain, Ovary
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Stewart Russell

Verified customer

投稿 Feb 18 2014

Thank you for contacting us.

The sequence homology between human and Platypus species seems quite OK for antibodies ab12337 and ab85084 cross reactivity. These antibodies may be tried for further tests. We can provide abrreview discount for th...

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Thank you for your enquiry. I am very pleased to hearthe customerwould like to make use of our testing discount offerand test ab12337 inIHC-P.

Discount code: 40213
Expiration date: 17th Nov 12

This code will give you: 1 freep...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

As requested, I have issued a free of charge replacement directly to your labwith the order nu...

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Thank you for contacting us.

Could you please reply to this email and send me the address of your lab so I can send a free of charge replacement of ab12337 lot GR31507-1?

Could you also please remind me the species you used the antibo...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Hela #F9 #Ms Testis #PC-12)
Loading amount 30 µg
Specification Hela #F9 #Ms Testis #PC-12
Gel Running Conditions Reduced Denaturing (7.5% SDS-PAGE)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Mr. Antibody Solutions

Verified customer

投稿 Mar 05 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Whole testis extract)
Loading amount 5 µg
Specification Whole testis extract
Gel Running Conditions Reduced Denaturing
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Aug 09 2007

Thank you for your enquiry. If my sequence comparison is correct (see link below for the next 24 hours) there is a significant degree of sequence similarity the immunogen used to raise ab12337 and PIWIL3 (NM_001008496). http://www.ebi.ac.uk/cgi...

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