製品の概要

  • 製品名Anti-PITX1 antibody
    PITX1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PITX1
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cow, Chimpanzee, Gorilla, Orangutan, Elephant
  • 免疫原

    Synthetic peptide corresponding to a sequence between N terminal amino acids 50-100 of Human PITX1. (NP_002644.3)

  • ポジティブ・コントロール
    • HeLa whole cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab70273 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2500 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).
IP Use at 1-4 µg/mg of lysate.

ターゲット情報

  • 機能May play a role in the development of anterior structures, and in particular, the brain and facies and in specifying the identity or structure of hindlimb.
  • 関連疾患Defects in PITX1 are a cause of congenital clubfoot (CCF) [MIM:119800]; also known as talipes equinovarus (TEV). Clubfoot is a congenital limb deformity defined as fixation of the foot in cavus, adductus, varus, and equinus (i.e. inclined inwards, axially rotated outwards, and pointing downwards) with concomitant soft tissue abnormalities. Clubfoot may occur in isolation or as part of a syndrome. Clubfoot appears to be a multifactorial trait.
  • 配列類似性Belongs to the paired homeobox family. Bicoid subfamily.
    Contains 1 homeobox DNA-binding domain.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BFT antibody
    • CCF antibody
    • Hindlimb expressed homeobox protein backfoot antibody
    • Hindlimb-expressed homeobox protein backfoot antibody
    • Homeobox protein PITX1 antibody
    • LBNBG antibody
    • Paired like homeodomain 1 antibody
    • Paired like homeodomain transcription factor 1 antibody
    • Paired-like homeodomain transcription factor 1 antibody
    • Pituitary homeo box 1 antibody
    • Pituitary homeobox 1 antibody
    • Pituitary otx related factor antibody
    • Pitx1 antibody
    • PITX1_HUMAN antibody
    • POTX antibody
    • PTX1 antibody
    see all

Anti-PITX1 antibody 画像

  • HeLa whole cell lysate (1 mg) was immunoprecipitated using ab70273 at 3 µg/mg lysate (lane 1) or an IgG control (lane 2). One quarter of the total IP reaction was loaded on to a gel and analysed by Western blot using ab70273 at 1 µg/ml. Bands were detected using chemiluminescence with an exposure time of 30 seconds.
  • All lanes : Anti-PITX1 antibody (ab70273) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 34 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)


    Exposure time : 3 minutes

Anti-PITX1 antibody (ab70273) 使用論文

This product has been referenced in:
  • Booy EP  et al. The RNA helicase RHAU (DHX36) suppresses expression of the transcription factor PITX1. Nucleic Acids Res 42:3346-61 (2014). Human . Read more (PubMed: 24369427) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab70273.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"