製品の概要

  • 製品名Anti-PITRM1 antibody
    PITRM1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PITRM1
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 1-188 of Human PITRM1 (AAH01150).

  • ポジティブ・コントロール
    • A549, HeLa, HepG2 and HCT116 cells and whole cell lysates; PC13 xenograft tissue.

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーpH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab111681 is purified by antigen-affinity chromatography.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab111681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 117 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

ターゲット情報

  • 機能ATP-independent protease that degrades mitochondrial transit peptides after their cleavage. Also degrades other unstructured peptides. Specific for peptides in the range of 10 to 65 residues. Able to degrade amyloid beta A4 (APP) protein when it accumulates in mitochondrion, suggesting a link with Alzheimer disease. Shows a preference for cleavage after small polar residues and before basic residues, but without any positional preference.
  • 組織特異性Widely expressed. Expressed at higher level in muscle and heart compared to brain, pancreas, liver, lung and placenta.
  • 配列類似性Belongs to the peptidase M16 family. PreP subfamily.
  • 翻訳後修飾The disulfide bond may lock the enzyme in a closed conformation under oxidized conditions, suggesting that it may participate in redox regulation of the enzyme.
  • 細胞内局在Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hMP1 antibody
    • hPreP antibody
    • KIAA1104 antibody
    • Metalloprotease 1 antibody
    • MGC138192 antibody
    • MGC141929 antibody
    • mitochondrial antibody
    • MP1 antibody
    • Pitrilysin metallopeptidase 1 antibody
    • Pitrilysin metalloproteinase 1 antibody
    • PITRM 1 antibody
    • PITRM1 antibody
    • PreP antibody
    • PreP peptidasome antibody
    • PREP_HUMAN antibody
    • Presequence protease antibody
    • Presequence protease mitochondrial antibody
    see all

Anti-PITRM1 antibody 画像

  • Anti-PITRM1 antibody (ab111681) at 1/1000 dilution + A549 whole cell lysate at 30 µg

    Predicted band size : 117 kDa
  • ab111681 at 1/200 dilution staining PITRM1 in methanol-fixed HeLa cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
  • ab111681 at 1/500 dilution staining PITRM1 in paraffin-embedded PC13 xenograft tissue by Immunohistochemistry.

Anti-PITRM1 antibody (ab111681) 使用論文

ab111681 has not yet been referenced specifically in any publications.

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There are currently no Abreviews or Questions for ab111681.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"