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RabMAb

Anti-PIST 抗体 [EPR4079] (ab109119)

製品の概要

  • 製品名
    Anti-PIST antibody [EPR4079]
    PIST 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR4079] to PIST
  • アプリケーション
    適用あり: ICC/IF, WB, IP, IHC-P, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • ポジティブ・コントロール
    • WB: A375, 293T, and PC-3 cell lysates and human prostate and fetal kidney tissue lysates. IHC-P: Human stomach tissue. ICC/IF: HeLa cells.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/50 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 51 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能
    Plays a role in intracellular protein trafficking and degradation. May regulate CFTR chloride currents and acid-induced ACCN3 currents by modulating cell surface expression of both channels. May also regulate the intracellular trafficking of the ADR1B receptor. May play a role in autophagy. Overexpression results in CFTR intracellular retention and degradation in the lysosomes.
  • 組織特異性
    Ubiquitously expressed.
  • 関連疾患
    Note=A chromosomal aberration involving GOPC is found in a glioblastoma multiforme sample. An intra-chromosomal deletion del(6)(q21q21) is responsible for the formation of GOPC-ROS1 chimeric protein which has a constitutive receptor tyrosine kinase activity.
  • 配列類似性
    Contains 1 PDZ (DHR) domain.
  • ドメイン
    The PDZ domain mediates interactions with FZD5, FZD8, ACCN3, GRID2, CLCN3 (By similarity). Mediates also interaction with CFTR and ADRB1.
    The coiled-coil region probably mediates association to membranes, targeting to the Golgi, and interactions with GOLGA3, and STX6. May also mediate interaction with RHOQ.
  • 細胞内局在
    Cytoplasm. Golgi apparatus membrane. Golgi apparatus > trans-Golgi network membrane. Cell junction > synapse. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Cell projection > dendrite. Enriched in synaptosomal and postsynaptic densities (PSD) fractions (By similarity). Expressed in cell bodies and dendrites of Purkinje cells (By similarity). Localized at the trans-Golgi network (TGN) of spermatids and the medulla of round spermatides.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CAL antibody
    • CFTR associated ligand antibody
    • CFTR-associated ligand antibody
    • dJ94G16.2 antibody
    • dJ94G16.2 PIST antibody
    • FIG antibody
    • Fused in glioblastoma antibody
    • Golgi associated PDZ and coiled coil motif containing antibody
    • Golgi associated PDZ and coiled coil motif containing protein antibody
    • Golgi-associated PDZ and coiled-coil motif-containing protein antibody
    • GOPC 1 antibody
    • GOPC antibody
    • GOPC_HUMAN antibody
    • GOPC1 antibody
    • OTTHUMP00000040403 antibody
    • PDZ protein interacting specifically with TC 10 antibody
    • PDZ protein interacting specifically with TC10 antibody
    • PDZ/coiled coil domain binding partner for the rho family GTPase TC 10 antibody
    • PDZ/coiled coil domain binding partner for the rho family GTPase TC10 antibody
    • PIST antibody
    • Protein interacting specifically with Tc 10 antibody
    • Protein interacting specifically with Tc10 antibody
    see all

画像

  • ab109119 stained HeLa cells. The cells were 4% formaldhyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab109119 at 1/100) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • All lanes : Anti-PIST antibody [EPR4079] (ab109119) at 1/1000 dilution

    Lane 1 : A375 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : Fetal kidney lysate
    Lane 4 : PC-3 cell lysate
    Lane 5 : Human prostate lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 51 kDa
  • Immunohistochemical staining of PIST expression in paraffin-embedded Human stomach tissue using ab109119 at 1/250 dilution.
  • ab109119 showing positive staining in Normal brain tissue.

  • ab109119 showing positive staining in Normal breast tissue.

  • ab109119 showing positive staining in Normal kidney tissue.

  • ab109119 showing positive staining in Normal liver tissue.

  • ab109119 showing positive staining in Normal ovary tissue.

参考文献

This product has been referenced in:
  • Belman JP  et al. Acetylation of TUG protein promotes the accumulation of GLUT4 glucose transporters in an insulin-responsive intracellular compartment. J Biol Chem 290:4447-63 (2015). Read more (PubMed: 25561724) »
  • Löffler MG  et al. Enhanced fasting glucose turnover in mice with disrupted action of TUG protein in skeletal muscle. J Biol Chem 288:20135-50 (2013). Read more (PubMed: 23744065) »

See all 2 Publications for this product

レビューと Q&A

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PIST has three isoforms: isoform 1 at 50.5 kDa and isoform 2 at 49.7 kDa (likely to appear as either a single thick band or a doublet), as well as isoform 3 at 35 kDa. These isoforms may be differentially expresse...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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