製品の概要

  • 製品名Anti-Pirin antibody
    Pirin 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to Pirin
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide:

    GSSKKVTLSVLSREC

    , corresponding to N terminal amino acids 2-15 of Human Pirin.

  • ポジティブ・コントロール
    • Human heart lysate. Lysate prepared from transfected HEK293 transiently expressing Pirin.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab21202 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 30-35 kDa (predicted molecular weight: 32 kDa).

ターゲット情報

  • 機能Possible transcriptional coregulator. May contribute to the regulation of cellular processes via its interaction with BCL3. May be required for efficient terminal myeloid maturation of hematopoietic cells. May play a role in the regulation of cell migration. May promote apoptosis when overexpressed. Has quercetin 2,3-dioxygenase activity (in vitro).
  • 組織特異性Highly expressed in a subset of melanomas. Detected at very low levels in most tissues (at protein level). Expressed in all tissues, with highest level of expression in heart and liver.
  • パスウェイFlavonoid metabolism; quercetin degradation.
  • 配列類似性Belongs to the pirin family.
  • 細胞内局在Nucleus. Cytoplasm. Predominantly localized in dot-like subnuclear structures. Cytoplasmic localization of PIR seems to positively correlate with melanoma progression.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 3-dioxygenase PIR antibody
    • Iron binding nuclear protein antibody
    • Pir antibody
    • PIR_HUMAN antibody
    • Pirin antibody
    • Probable quercetin 2 antibody
    • probable quercetin 2,3-dioxygenase PIR antibody
    • Probable quercetinase antibody
    see all

Anti-Pirin antibody 画像

  • Anti-Pirin antibody (ab21202) at 0.3 µg/ml + Human Heart lysate (35µg protein in RIPA buffer).
    Developed using the ECL technique

    Predicted band size : 32 kDa
    Observed band size : 30-35 kDa (why is the actual band size different from the predicted?)
  • Anti-Pirin antibody (ab21202) at 1 µg/ml + lysate prepared from transfected HEK293 cells transiently over-expressing Pirin.

    Predicted band size : 32 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 32 kDa. We are unsure as to the identity of these extra bands.

Anti-Pirin antibody (ab21202) 使用論文

This product has been referenced in:
  • Brzóska K  et al. Basal PIR expression in HeLa cells is driven by NRF2 via evolutionary conserved antioxidant response element. Mol Cell Biochem 389:99-111 (2014). WB ; Human . Read more (PubMed: 24390086) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"