Anti-PIP5K3 (phospho S307) 抗体 (ab62467)

製品の概要

  • 製品名
    Anti-PIP5K3 (phospho S307) antibody
    PIP5K3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PIP5K3 (phospho S307)
  • 特異性
    ab62467 detects endogenous levels of PIP5K3 only when phosphorylated at serine 307.
  • アプリケーション
    適用あり: ELISA, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human PIP5K3 around the phosphorylation site of serine 307 (S-A-SP-I-T).

  • ポジティブ・コントロール
    • Human brain tissue and HeLa cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    ab62467 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/10000.
ICC/IF 1/500 - 1/1000.
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能
    The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Catalyzes the phosphorylation of phosphatidylinositol 3-phosphate on the fifth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 3,5-bisphosphate. Required for endocytic-vacuolar pathway and nuclear migration. Plays a role in the biogenesis of endosome carrier vesicles (ECV)/ multivesicular bodies (MVB) transport intermediates from early endosomes.
  • 関連疾患
    Corneal dystrophy, fleck
  • 配列類似性
    Contains 1 DEP domain.
    Contains 1 FYVE-type zinc finger.
    Contains 1 PIPK domain.
  • 細胞内局在
    Endosome membrane. Mainly associated with membranes of the late endocytic pathway.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1 phosphatidylinositol 3 phosphate 5 kinase antibody
    • 1 phosphatidylinositol 4 phosphate 5 kinase antibody
    • 1-phosphatidylinositol 3-phosphate 5-kinase antibody
    • CFD antibody
    • epididymis luminal protein 37 antibody
    • FAB1 antibody
    • FAB1, S. cerevisiae, homolog of antibody
    • FYV1_HUMAN antibody
    • FYVE finger containing phosphoinositide kinase antibody
    • FYVE finger-containing phosphoinositide kinase antibody
    • KIAA0981 antibody
    • MGC40423 antibody
    • p235 antibody
    • Phosphatidylinositol 3 phosphate 5 kinase type III antibody
    • Phosphatidylinositol 3 phosphate/phosphatidylinositol 5 kinase type III antibody
    • Phosphatidylinositol 3-phosphate 5-kinase antibody
    • Phosphatidylinositol 3-phosphate 5-kinase type III antibody
    • Phosphatidylinositol 4 phosphate 5 kinase type III antibody
    • Phosphoinositide kinase, FYVE finger containing antibody
    • PIKfyve antibody
    • PIP5K antibody
    • PIP5K3 antibody
    • PIPkin III antibody
    • PIPkin-III antibody
    • PtdIns(4)P 5 kinase antibody
    • Type III PIP kinase antibody
    • ZFYVE29 antibody
    • Zinc finger, FYVE domain containing 29 antibody
    see all

Anti-PIP5K3 (phospho S307) antibody 画像

  • ab62467, at 1/50 dilution, staining PIP5K3 in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide.
  • ab62467, at 1/500 dilution, staining PIP5K3 in HeLa cells by Immunofluorescence, in the absence or presence of the immunising peptide.

Anti-PIP5K3 (phospho S307) antibody (ab62467) 使用論文

ab62467 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Mammary Epithelial)
Loading amount
50 µg
Specification
Mammary Epithelial
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Odyssey Blocking Buffer (Li-COR) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 30%
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投稿 Jul 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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