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Our Abpromise guarantee covers the use of ab55597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: PIP5K2 alpha knockout HAP1 cell lysate (20 µg)
Lane 3: K562 cell lysate (20 µg)
Lane 4: Jurkat cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab55597 observed at 50 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab55597 was shown to recognize PIP5K2 alpha when PIP5K2 alpha knockout samples were used, along with additional cross-reactive bands. Wild-type and PIP5K2 alpha knockout samples were subjected to SDS-PAGE. ab55597 and ab181602 (loading control to GAPDH) were diluted at 1 μg/mL and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
ab55597 has not yet been referenced specifically in any publications.