アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。
Recombinant full length protein corresponding to Human PINK1.
Our Abpromise guarantee covers the use of ab75487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|WB||1/100 - 1/500. Predicted molecular weight: 63 kDa.|
Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling PINK1 (green) with ab75487 at a dilution of 1/25. An Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/400). Cytoplasmic actin was counterstained with Alexa Fluor® 555-conjugated with Phalloidin (red).
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling PINK1 with ab75487. A peroxidase-conjugated anti-mouse IgG was used as the secondary antibody, followed by DAB staining.
Immunocytochemistry/Immunofluorescence analysis of PC12 cells labelling PINK1 with ab75487. Cells were fixed with paraformaldehyde, permeabilization with 0.3X Triton X-100 and blocked with 10% serum for 1 hour at 4°C. Cells were incubated with the primary antibody at 2 µg/ml for 24 hours at 4°C. An Alexa Fluor® 488-conjugated anti-mouse (1/1000) was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab75487 has not yet been referenced specifically in any publications.