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Recombinant full length protein corresponding to Human PINK1.
Our Abpromise guarantee covers the use of ab75487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/100.|
|ICC/IF||Use a concentration of 1 - 2 µg/ml.|
|WB||1/100 - 1/500. Predicted molecular weight: 63 kDa.|
Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/Immunofluorescence analysis of PC-12 cells labelling PINK1 (green) with ab75487 at a dilution of 1/25. An Alexa Fluor® 488-conjugated goat anti-mouse IgG was used as the secondary antibody (1/400). Cytoplasmic actin was counterstained with Alexa Fluor® 555-conjugated with Phalloidin (red).
Immunohistochemical analysis of paraffin-embedded Human heart section, labelling PINK1 with ab75487 (1/25). An undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunocytochemistry/Immunofluorescence analysis of PC12 cells labelling PINK1 with ab75487. Cells were fixed with paraformaldehyde, permeabilization with 0.3X Triton X-100 and blocked with 10% serum for 1 hour at 4°C. Cells were incubated with the primary antibody at 2 µg/ml for 24 hours at 4°C. An Alexa Fluor® 488-conjugated anti-mouse (1/1000) was used as the secondary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of paraffin-embedded Human stomach section, labelling PINK1 with ab75487 (1/25). An undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
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