Anti-Pin1 (phospho S16) 抗体 [EP1480Y] (ab62378)

製品の概要

  • 製品名Anti-Pin1 (phospho S16) antibody [EP1480Y]
    Pin1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1480Y] to Pin1 (phospho S16)
  • 特異性This antibody detects Pin1 phosphorylated at Serine 16.
  • アプリケーション適用あり: WB, ICCmore details
    適用なし: Flow Cyt or IP
  • 種交差性
    交差種: Human
  • 免疫原

    Phospho specific peptide corresponding to residues surround Serine 16 of human Pin1.

  • ポジティブ・コントロール
    • HeLa cell lysate.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab62378 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ICC 1/100 - 1/250.
  • 追加情報Is unsuitable for Flow Cyt or IP.
  • ターゲット情報

    • 機能Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
    • 配列類似性Contains 1 PpiC domain.
      Contains 1 WW domain.
    • ドメインThe WW domain is required for the interaction with STIL and KIF20B.
    • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DOD antibody
      • DODO, Drosophila, homolog of antibody
      • FLJ40239 antibody
      • FLJ77628 antibody
      • MGC10717 antibody
      • NIMA interacting 1 antibody
      • Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody
      • Peptidyl prolyl cis/trans isomerase NIMA interacting antibody
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody
      • Peptidyl-prolyl cis-trans isomerase pin1 antibody
      • Peptidylprolyl cis/trans isomerase NIMA interacting 1 antibody
      • Pin 1 antibody
      • Pin1 antibody
      • PIN1_HUMAN antibody
      • PPIase Pin1 antibody
      • Prolyl isomerase antibody
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
      • Protein NIMA interacting 1 antibody
      • Rotamase Pin1 antibody
      • UBL 5 antibody
      • UBL5 antibody
      see all

    Anti-Pin1 (phospho S16) antibody [EP1480Y] 画像

    • All lanes : Anti-Pin1 (phospho S16) antibody [EP1480Y] (ab62378) at 1/500 dilution

      Lane 1 : HeLa cell lysate (untreated)
      Lane 2 : HeLa cell lysate (treated with FBS + Calyculin A)

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat anti-Rabbit HRP conjugated at 1/2000 dilution

      Predicted band size : 18 kDa
      Observed band size : 18 kDa

    Anti-Pin1 (phospho S16) antibody [EP1480Y] (ab62378) 使用論文

    ab62378 has not yet been referenced specifically in any publications.

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Liver)
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
    Permeabilization Yes - Wash Buffer from Dako with Tween
    Specification Liver
    Fixative Paraformaldehyde
    Username

    Mr. Rudolf Jung

    Verified customer

    投稿 Oct 20 2015

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"