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RabMAb

Anti-Pin1 抗体 [EP1479Y] (ab76309)

製品の概要

  • 製品名
    Anti-Pin1 antibody [EP1479Y]
    Pin1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1479Y] to Pin1
  • アプリケーション
    適用あり: WB, ICC, Flow Cytmore details
    適用なし: IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    within Human Pin1 aa 50-150 (N terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • 293 cell lysate.
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
ICC 1/100 - 1/250.
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報
    Is unsuitable for IHC-P or IP.
  • ターゲット情報

    • 機能
      Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
    • 配列類似性
      Contains 1 PpiC domain.
      Contains 1 WW domain.
    • ドメイン
      The WW domain is required for the interaction with STIL and KIF20B.
    • 翻訳後修飾
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DOD antibody
      • DODO, Drosophila, homolog of antibody
      • FLJ40239 antibody
      • FLJ77628 antibody
      • MGC10717 antibody
      • NIMA interacting 1 antibody
      • Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody
      • Peptidyl prolyl cis/trans isomerase NIMA interacting antibody
      • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody
      • Peptidyl-prolyl cis-trans isomerase pin1 antibody
      • Peptidylprolyl cis/trans isomerase NIMA interacting 1 antibody
      • Pin 1 antibody
      • Pin1 antibody
      • PIN1_HUMAN antibody
      • PPIase Pin1 antibody
      • Prolyl isomerase antibody
      • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
      • Protein NIMA interacting 1 antibody
      • Rotamase Pin1 antibody
      • UBL 5 antibody
      • UBL5 antibody
      see all

    画像

    • Anti-Pin1 antibody [EP1479Y] (ab76309) at 1/500 dilution + 293 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 18 kDa
      Observed band size : 18 kDa
    • Overlay histogram showing HeLa cells stained with ab76309 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76309, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    参考文献

    This product has been referenced in:
    • Xu T  et al. Loss of Pin1 Suppresses Hedgehog-Driven Medulloblastoma Tumorigenesis. Neoplasia 19:216-225 (2017). Read more (PubMed: 28167297) »
    • Guito J  et al. The cellular peptidyl-prolyl cis/trans isomerase Pin1 regulates reactivation of Kaposi's sarcoma-associated herpesvirus from latency. J Virol 88:547-58 (2014). IP . Read more (PubMed: 24173213) »

    See all 4 Publications for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Rat Ovary sections)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: 0.05% Protease treatment for 5 mins @ 37' C
    Permeabilization
    No
    Specification
    Rat Ovary sections
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 1.5%
    Fixative
    Formaldehyde
    Username

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    Verified customer

    投稿 Dec 04 2015

    The Pin1 antibody ab76309 has been tested for IF staining of cell cultures. We found that a dilution range of 1/100-1/250 produced the best result, with minimal background, using a fluorophore-conjugated secondary antibody for detection. But you may wa...

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    Vielen Dank für Ihre Anfrage.

    Ich fürchte, dass dieser Antikörper nicht für Sie geeignet ist, da er die Aminosaueren in Position AS XX-YY erkennt. Wenn Sie mir allerdings mitteilen, für welche Applikation Sie den Antik...

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    Thank you for contacting us.
    The price for product is same for Poland and Portugal and is given below;
    Product price = Euros 370
    Delivery price = Euros 42
    I hope this information is helpful to you. Please do not hesitate to contact us...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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