製品の概要

  • 製品名
    Anti-Pin1 antibody [3G8]
    Pin1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [3G8] to Pin1
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1/163 of Human Pin1

  • ポジティブ・コントロール
    • 293T, HeLa and Jurkat cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 18 kDa.
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Catalyzing pSer/Thr-Pro cis/trans isomerizations.
  • 配列類似性
    Contains 1 PpiC domain.
    Contains 1 WW domain.
  • ドメイン
    The WW domain is required for the interaction with STIL and KIF20B.
  • 翻訳後修飾
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DOD antibody
    • DODO, Drosophila, homolog of antibody
    • FLJ40239 antibody
    • FLJ77628 antibody
    • MGC10717 antibody
    • NIMA interacting 1 antibody
    • Peptidyl prolyl cis trans isomerase NIMA interacting 1 antibody
    • Peptidyl prolyl cis/trans isomerase NIMA interacting antibody
    • Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 antibody
    • Peptidyl-prolyl cis-trans isomerase pin1 antibody
    • Peptidylprolyl cis/trans isomerase NIMA interacting 1 antibody
    • Pin 1 antibody
    • Pin1 antibody
    • PIN1_HUMAN antibody
    • PPIase Pin1 antibody
    • Prolyl isomerase antibody
    • Protein (peptidylprolyl cis/trans isomerase) NIMA interacting 1 antibody
    • Protein NIMA interacting 1 antibody
    • Rotamase Pin1 antibody
    • UBL 5 antibody
    • UBL5 antibody
    see all

画像

  • All lanes : Anti-Pin1 antibody [3G8] (ab38933) at 1/500 dilution

    Lane 1 : 293T cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    goat anti-mouse secondary antibody conjugated to HRP
    Developed using the ECL technique

    Predicted band size : 18 kDa
    Observed band size : 16 kDa (why is the actual band size different from the predicted?)

プロトコール

参考文献

ab38933 has not yet been referenced specifically in any publications.

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