The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 120 kDa (predicted molecular weight: 97 kDa).
Use at 2-5 µg/mg of lysate.
Catalyzes the 2 serial methylation steps for the conversion of the 7-monomethylguanosine (m(7)G) caps of snRNAs and snoRNAs to a 2,2,7-trimethylguanosine (m(2,2,7)G) cap structure. The enzyme is specific for guanine, and N7 methylation must precede N2 methylation. Hypermethylation of the m7G cap of U snRNAs leads to their concentration in nuclear foci, their colocalization with coilin and the formation of canonical Cajal bodies (CBs). Plays a role in transcriptional regulation.
Ubiquitously expressed. High expression in heart, skeletal muscle, kidney, liver and placenta.
Belongs to the methyltransferase superfamily. Trimethylguanosine synthase family.
Cytoplasm. Nucleus > Cajal body. A 90 kDa isoform is found in the nucleus while a 55 kDa isoform is found in the cytoplasm and colocalizes with the tubulin network.
There are multiple additional bands. We are unsure as to the identity of these extra bands.
Immunoprecipitation - PIMT antibody (ab70559)
Detection of Human PIMT by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab70559 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.