製品の概要

  • 製品名Anti-PIM3 antibody - Carboxyterminal end
    PIM3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PIM3 - Carboxyterminal end
  • アプリケーション適用あり: WB, IHC-P, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human PIM3 aa 259-288 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 415116

  • ポジティブ・コントロール
    • WB: Mouse kidney tissue lysate IHC-P: Human hepatocarcinoma tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71321 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/100 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 36 kDa).
IHC-P 1/10 - 1/50.
ELISA 1/1000.

ターゲット情報

  • 機能Able to phosphorylate CDKN1B in vitro. Involved in cell cycle progression and suppression of apoptosis. Implicated in proliferation of human hepatoma cell lines.
  • 組織特異性Widely expressed. No expression in colon, thymus, and small intestine. Expressed in human hepatoma cell lines but not in normal liver tissues. Expressed in colon cancer samples, particularly at the less advanced stages of cancer development.
  • 配列類似性Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
    Contains 1 protein kinase domain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Kid 1 antibody
    • Kid1 antibody
    • Kinase induced by depolarization antibody
    • ONCOGENE PIM3 antibody
    • PIM 3 antibody
    • Pim 3 oncogene antibody
    • Pim-3 proto-oncogene, serine/threonine kinase antibody
    • PIM3 antibody
    • Pim3 oncogene antibody
    • PIM3_HUMAN antibody
    • Protein kinase Kid 1 antibody
    • Protein kinase Kid1 antibody
    • Serine/threonine kinase Pim 3 antibody
    • Serine/threonine kinase Pim3 antibody
    • Serine/threonine protein kinase Pim 3 antibody
    • Serine/threonine protein kinase Pim3 antibody
    • Serine/threonine-protein kinase Pim-3 antibody
    see all

Anti-PIM3 antibody - Carboxyterminal end 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocarcinoma tissue sections labeling PIM3 - Carboxyterminal end with ab71321 at 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 37°C. Heat mediated antigen retrieval was performed using a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A Peroxidase-conjugated Goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Anti-PIM3 antibody - Carboxyterminal end (ab71321) at 1/1000 dilution + mouse kidney tissue lysate at 12.5 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

    Predicted band size : 36 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.

    Blocking/Dilution buffer: 5% NFDM/TBST.

Anti-PIM3 antibody - Carboxyterminal end (ab71321) 使用論文

This product has been referenced in:
  • Selmi T  et al. ZFP36 expression impairs glioblastoma cell lines viability and invasiveness by targeting multiple signal transduction pathways. Cell Cycle 11:1977-87 (2012). WB ; Human . Read more (PubMed: 22544323) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"